土壤有机物和营养物质的可用性会影响低碳能源的适用性,在土壤中增加了Dehalococcoides的土壤
Lingyu Meng1, Ryuya Tomita1, Tomoki Yoshida1
1Department of Civil Engineering, Nagoya Institute of Technology (Nitech), Nagoya 466-8555, Japan.
Journal of hazardous materials
|August 17, 2023
概括
在土壤中,当添加特定的低碳能源,如乙醇,酸盐或乳酸盐时,dehalokoccoides生物增量有效地脱三乙烯 (TCE). 土壤有机物和蛋白质含量是成功去除TCE的关键因素.
科学领域:
- 环境微生物学 环境微生物学
- 生物修复是一种生物修复.
- 微生物生态学 微生物生态学
背景情况:
- 脱可可化物对于三乙烯 (TCE) 完全脱至关重要.
- 使用Dehalococcoides进行生物增量可以减少环境干扰,但其在土壤中的适用性尚不确定.
- 确定污染土壤中Dehalococcoides的最佳条件对于有效的生物修复至关重要.
研究的目的:
- 评估Dehalococcoides生物增量在TCE污染土壤修复中的适用性.
- 评估七种低碳能源作为德哈洛科科伊德电子捐赠者的有效性.
- 为了确定土壤特性对TCE脱由Dehalococcoides的影响.
主要方法:
- 用Dehalococcoides和七个不同的电子捐赠者 (甲醇,甲酸盐,乙酸盐,乙醇,乳酸盐,酸盐,酸盐) 建立了土壤微观世界.
- 随着时间的推移,对三乙烯 (TCE) 脱活性进行了监测.
- 使用PICRUSt2从16S rRNA基因序列分析了功能性基因配置文件.
- 测量了土壤有机物和蛋白质度.
主要成果:
- 在111-180天内补充乙醇,甲酸盐或乳酸盐的土壤中观察到显著的TCE脱.
- 功能性基因配置文件与Dehalococcoides生物增大适用性没有相关性.
- 在高有机物 (>3.2%) 和蛋白质度 (>1.6μg/mL) 的土壤中实现了完全脱.
结论:
- 乙醇,甲酸盐和乳酸盐是适合在土壤中通过Dehalococcoides介导的TCE脱的低碳能源.
- 土壤有机物和营养物质的可用性是影响Dehalococcoides生物增量成功的关键因素.
- 这项研究提供了实验证据,支持使用特定的电子捐赠者和有利的土壤条件来有效地进行TCE生物修复.
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