铁和调节碳转化在甲类微藻系统中
Baorui Zhang1, Chen Cai2, Yan Zhou3
1Interdisciplinary Graduate Program, Nanyang Technological University, 61 Nanyang Drive, 637335, Singapore; Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
The Science of the total environment
|August 17, 2023
概括
铁和是甲类植物和微藻 (MOB-MG) 共同培养的关键营养素. 优化铁能促进生长和生物气转化,而会影响生长和蛋白质含量,这对于有效的生物气价值化至关重要.
科学领域:
- 生物技术和环境科学 生物技术和环境科学
- 微生物生态学 微生物生态学
- 生物工艺工程 生物工艺工程
背景情况:
- 甲基和微藻 (MOB-MG) 共同培养对生物气的价值化有希望.
- 了解营养调节对于优化MOB-MG系统性能至关重要.
- 目前关于铁和在MOB-MG培动态中的作用的知识是有限的.
研究的目的:
- 研究铁和的可用性对MOB-MG种植生长,生物气转化和生物质组成的影响.
- 确定营养成分的修改如何影响MOB-MG系统内的微生物社区结构.
- 评估异质型细菌在共同培养系统中的作用.
主要方法:
- 使用合成沼气从废弃活性污泥中建立高蛋白MOB-MG共养殖.
- 控制改变铁和含量,以评估它们对养殖业绩效的影响.
- 分析特定生长率,生物气转化效率和生物质组成 (蛋白质,脂质).
- 微生物社区分析使用16S rRNA基因测序来识别MOB和其他种群的变化.
主要成果:
- 增加的铁可用性显著提高了特定生长率 (0.18到0.62天-1),以及生物气转化 (26.81到106.57毫克-C L-1天-1).
- 的限制降低了特定生长率 (0.64~0.28天−1) 和蛋白质含量 (0.51~0.31g/g生物质),而脂质含量保持稳定.
- 铁和的修正将MOB种群从Methylococcus/Methylosinus转移到Methylocystis,而Chlorella始终主导着微藻.
- 存在非甲性异质体,但不会影响系统性能,可能会增强碳转化.
结论:
- 铁和是MOB-MG养殖表现的关键调节剂,影响生长,生物气转化和生物质质量.
- 优化营养供应,特别是铁,对于高效的生物气利用和生物质生产至关重要.
- 微生物群落结构,包括MOB和异构菌,对生物气利用过程的整体效率起着作用.
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