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跨多个组织的循环RNA转录组揭示了骨肌肉特定的circPSME4调节肌体发生

Mu Zeng¹, Shanying Yan¹, Peng Yang²

¹Kunpeng Institute of Modern Agriculture at Foshan, Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Foshan 528226, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China; Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.

International journal of biological macromolecules

|August 17, 2023

概括

这项研究揭示了中国和西方猪中的新型循环RNA (circRNAs),鉴定了许多差异表达和组织特定的circRNA. 骨肌特异性circPSME4促进肌细胞的发育,为动物育种和生物医学研究提供了洞察力.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

西方商业猪和中国本地品种之间存在显著的表型差异,例如肉类生产和生长速度等特征.
对比转录组学已经确定了候选基因,但环形RNAs (circRNAs) 在猪组织发育中的作用仍然在很大程度上未被探索.

研究的目的:

在卢川 (中国本地品种) 和杜洛克 (西方商业品种) 猪的十种组织中对circRNA表达特征进行全面的全基因组分析.
确定差异表达和组织特异性的circRNA,并研究新型circRNA在骨肌肉发育中的功能.

主要方法:

从卢chuan和Duroc猪的十种组织中对全基因组circRNA表达的分析.
鉴定和注释circRNAs,差异表达分析和丰富分析.
竞争性内源性RNA (ceRNA) 网络的构建和myoblasts中circPSME4的功能分析.

主要成果:

总共有56,254个circRNAs被确定,其中42.9%是新发现的,33.7%显示品种之间的差异表达.
确定了538种组织特异性circRNA,主要在脑和骨肌肉组织中.
发现骨肌特异性circPSME4能够调节肌细胞增殖和分化,作为骨肌发育的新型调节剂.

结论:

这项研究为养猪和生物医学研究提供了 circRNAs 的宝贵资源.
不同表达的circRNAs可能有助于中国本地和西方商业猪之间的表型差异.
circPSME4被确定为骨肌肉发育的新型调节剂,在改善肉类生产特征方面具有潜在的应用.

关键词:

在CircPSME4中使用.循环RNAs 是一个循环RNA.多个组织的多个组织.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪骨架肌肉是一个骨肌肉.

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