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用非正规氨基酸广泛破坏遗传密码退化

Clinton A L McFeely^1,2, Bipasana Shakya^1,2, Chelsea A Makovsky^1,2

¹Department of Chemistry, Virginia Commonwealth University, 1001 W Main St., Richmond, VA, 23284, USA.

Nature communications

|August 17, 2023

概括

使用感官密码重新分配的遗传密码扩展由超精确的核糖体增强. 这一突破使编码更多的氨基酸成为可能,为广泛的基因编码工程铺平了道路.

科学领域:

合成生物学合成生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

遗传密码扩展 (GCE) 能够在合成生物和药物发现中实现新型应用.
感官密码子重新分配 (SCR) 是一种GCE策略,用于打破密码子退化,但受到tRNA修改和核糖体摇摆读数的限制.
了解编码子-tRNA相互作用对于推进SCR至关重要.

研究的目的:

开发一种测试方法,用于评估特定编码子的氨基-tRNA竞争.
为了研究超精确的核糖体突变对摇摆读数和SCR的影响.
证明广泛和可预测的SCR的潜力.

主要方法:

开发了一种新的测定方法来测量氨基-tRNA对子结合的竞争.
利用超精确的核糖体突变来减少非特异性tRNA结合.
将这些突变物与未经修改的tRNA配对,以实现感官密码子的重新分配.

主要成果:

开发的试验有效地评估了编码子-tRNA配对动态.
超精确的核糖体显著减少波动读数.
获得了广泛和可预测的SCR,在两个编码盒中的九个编码体中编码了七种不同的氨基酸.

结论:

遗传密码具有比以前理解的更多的重新分配潜力.
超精确的核糖体与未经修改的tRNA相结合,促进了广泛的SCR.
这项工作为全面的基因编码工程开辟了新的途径.

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