Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Global approaches to infectious disease surveillance and modeling.

Nature medicine·2026
Same author

Genomic epidemiology of the 2025 mpox epidemic in Sierra Leone.

Nature medicine·2026
Same author

Environmental surveillance of pathogens in Africa.

Applied and environmental microbiology·2026
Same author

Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2026
Same author

Tensor cores unlock efficient and lower-energy massive parallelization on phylogenetic trees.

Systematic biology·2026
Same author

Deciphering the etiology of the 2024 outbreak of undiagnosed febrile illness in Panzi, Democratic Republic of the Congo.

Nature medicine·2026

相关实验视频

Updated: Jul 19, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K

使用嵌套PCR和纳米孔序列测序进行敏感的脊髓灰质炎病毒检测:一项前性验证研究.

Alexander G Shaw1, Tresor Kabeya Mampuela2,3, Emmanuel Lokilo Lofiko3

  • 1MRC Centre for Global Infectious Disease Analysis, School of Public Health, Imperial College London, London, UK. a.shaw@imperial.ac.uk.

Nature microbiology
|August 17, 2023
PubMed
概括

直接分子检测和纳米孔序列 (DDNS) 在刚果民主共和国提供更快的脊髓灰质炎病毒检测. 这种方法证实了脊髓灰质炎病毒病例和疫情比传统方法快得多,有助于根除疫情.

更多相关视频

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.8K
Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K

相关实验视频

Last Updated: Jul 19, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.8K
Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K

科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 公共卫生 公共卫生

背景情况:

  • 消灭脊髓灰质炎病毒需要及时检测疫情,但刚果民主共和国目前的黄金标准方法的中位检测时间为30天.
  • 直接分子检测和纳米孔序列测定 (DDNS) 为便样本中快速识别脊髓灰质炎病毒提供了一个有希望的替代方案.

研究的目的:

  • 未来评估DDNS在刚果民主共和国疑似脊髓灰质炎病例和接触者的便样本中检测脊髓灰质炎病毒的性能.
  • 将DDNS的速度和准确性与黄金标准检测方法进行比较.

主要方法:

  • 使用DDNS,对疑似脊髓灰质炎病例和接触者的便样本进行前性测试.
  • 将DDNS结果与包括细胞培养,定量PCR和桑格测序在内的黄金标准方法进行比较.
  • 分析两种方法的检测时间和序列相似性.

主要成果:

  • DDNS检测到2.7%的样本 (62/2,339) 中有脊髓灰质炎病毒,而黄金标准则为2.2% (51/2,339).
  • 在常规监测下,DDNS在平均7天内提供了病例确认.
  • 通过DDNS,可以更早地确认3个2型血清型流传的疫苗衍生的脊髓灰质炎疫情,平均23天 (范围6-30天).
  • DDNS和黄金标准方法之间的平均序列相似性为99.98%.

结论:

  • DDNS是一种可行且有效的方法,用于在国家实验室快速检测脊髓灰质炎病毒.
  • 实施DDNS可以显著加快疫情检测和应对,有助于消除脊髓灰质炎病毒的努力.
  • 与传统的脊髓灰质炎病毒检测方法相比,DDNS在处理时间上有了显著的改进.