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在印度,Phloeospora maculans的形态文化,病理和分子变异性导致了木 (Morus物种) 的叶子斑点

Zakiya Saif¹, Sabiha Ashraf², Mehraj D Shah³

¹College of Temperate Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.

Molecular biology reports

|August 17, 2023

概括

叶子斑病在木中,由Phloeospora maculans引起,在克什米尔显示出高度的多样性. 了解这个病原体的理解.

科学领域:

植物病理学植物病理学
菌类学菌类学是指菌类学.
丝工业研究丝工业研究

背景情况:

叶斑病 (LSD) 是由Phloeospora maculans引起的,对印度查和克什米尔的丝行业构成重大威胁.
有效的疾病管理需要了解病原体的种群结构和多样性.

研究的目的:

为了调查影响克什米尔山谷木的Phloeospora maculans的种群结构和遗传多样性.
用传统和分子技术来评估变异性.

主要方法:

采用了传统方法:对27个Phloeospora maculans分离物的形态,文化和病理特征.
利用分子方法,特别是简单序列重复 (ISSR) 标记,分析遗传变异性.
从查和克什米尔的斯里纳格,班迪波拉和巴拉穆拉地区收集的隔离物.

主要成果:

在基于形态文化和病理特征的Phloeospora maculans分离物中观察到很高的变异性.
ISSR标记物显示出显著的多态性,证实了它们适用于评估遗传多样性的适用性.
遗传学分析将27个分离物分为两个不同的种群,在种群内具有很高的遗传变异 (98.91%) 和种群之间的低变异 (1.08%).
种群之间的低遗传差异是由0.01.01的Fst值表明的.

结论:

在克什米尔山谷内,在形态,文化,病理和分子层面上存在Phloeospora maculans的高度多样性.
观察到的种群结构凸显了为在木中成功管理LSD而进行详细研究的必要性.
调查结果为该地区制定有针对性的疾病控制战略提供了关键的见解.

关键词:

这就是ISSR的ISSR.叶子上的斑点.莫鲁斯 (Morus) 种类的动物.这种植物是Phloeospora maculans.聚合酶连锁反应的发生.可变性的变化.

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