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Cucurbita moschata的一种与病变发生相关的蛋白质1对粉状菌感染有反应

Wei-Li Guo^1,2, He-Lian Yang¹, Jin-Peng Zhao¹

¹School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, China.

Frontiers in genetics

|August 18, 2023

概括

这项研究表明,南瓜CmPR1基因增强了植物对粉 (PM) 的抵抗力. 过度表达CmPR1可以提高植物对PM病原体的防御能力,为作物改善提供了一个新的策略.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

粉 (PM) 在全球显著降低了南瓜 (Cucurbita moschata) 的产量.
这种真菌Podosphaera xanthii导致南瓜PM.
一个耐PM的南瓜产生了一种与病变发生相关的蛋白1 (PR1) 同类蛋白 (CmPR1).

研究的目的:

研究南瓜CmPR1基因在赋予对PM的耐药性的作用.
确定CmPR1对植物防御机制对PM病原体的影响.

主要方法:

亚细胞局部化试验,以确定CmPR1在植物细胞中的位置.
在各种压力治疗下进行基因表达分析 (P. xanthii,乙烯,JA,NaCl,H2O2,SA).
产生和分析过度表达CmPR1的转基因烟草植物,以评估PM耐药性.

主要成果:

CmPR1是一种细胞质蛋白.
CmPR1表达是由P. xanthii,ET,JA和NaCl诱导的,但是由H2O2和SA抑制的.
烟草中的CmPR1过度表达增强了对Golovinomyces cichoracearum的抵抗力,可能是通过诱导细胞死亡和H2O2积累.
CmPR1通过酸 (SA) 信号通路积极调节颗粒电阻.

结论:

在Cucurbita moschata中,CmPR1充当了防御反应基因.
可以利用CmPR1开发耐PM的作物品种.

关键词:

黄瓜 (Cucurbita moschata) 是一种植物.在 PR-1 中使用 PR-1.功能分析是一种功能分析.粉状菌是一种粉状菌.转基因烟草是一种转基因烟草.

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