相关实验视频

Updated: Jul 19, 2025

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

关于SSR标记物和SNP的数据从Parvocalanus crassirostris的转录组中过

Nazima Habibi¹, Saif Uddin¹, Montaha Behebehani¹

¹Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, Kuwait.

|August 18, 2023

概括

海洋酸化和变暖威胁着足,这是海洋食物网的重要组成部分. 这项研究揭示了使用转录组测序的三代Parvocalanus crassirostris的遗传变异,识别了保护的新标记.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
气候变化科学气候变化科学

背景情况:

在海洋食物网中,Calanoid copepods 是重要的主要消费者.
海洋酸化 (OA) 和海洋变暖 (OW) 对脚动物种群构成重大威胁.
了解对环境变化的遗传反应对于海洋生态系统健康至关重要.

研究的目的:

为了研究三代跨越的Parvocalanus crassirostris的遗传变异.
识别用于评估多样性和保护需求的遗传标记.
为了解决缺乏关于足对气候变化反应的分子研究的问题.

主要方法:

使用Illumina HiSeq (2 x 100 bp配对结尾) 的转录组测序.
使用Trinity 2.6.6.6 的数据组合 2.6.6.
使用MISA和Primer 3识别单核酸多态 (SNP) 和简单序列重复 (SSR).

主要成果:

发现了413,890个SNP,其频率为每千亿2.8次.
已经确定了15222个SSR.
设计了28,944对用于潜在遗传标记的原料对.

结论:

已识别的SNP和SSR可以作为Parvocalanus crassirostris.的有价值的遗传标记.
这些标记将有助于未来对遗传多样性和保护工作的研究.
提供了基本的分子数据,以了解足动物适应气候变化的情况.

关键词:

在Indelsels中,我们可以看到.它们是初级的.简单的序列重复重复.单个核酸的多态性.转录组转录组就是一个转录组.

更多相关视频

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...