亚MIC抗生素对河流生物膜社区的微生物群,抗体和结构产生影响
Gabriela Flores-Vargas1, Darren R Korber1, Jordyn Bergsveinson2
1Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Frontiers in microbiology
|August 18, 2023
概括
持续暴露于低抗生素度 (低于最小的抑制度) 会促进河流生物膜的抗生素耐药性. 这项研究揭示了微生物社区结构和功能的转变,这是由于MIC以下的抗生素暴露.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 水生生态系统 水生生态系统
背景情况:
- 抗生素的低于最低抑制度 (sub-MICs) 可以影响水生环境,但其影响尚未完全理解.
- 生物膜社区在水生生态系统中至关重要,可以庇护各种微生物社区和耐药基因.
研究的目的:
- 研究慢性亚MIC抗生素暴露对河流生物膜社区的影响.
- 分析微生物组,抗体组,病毒组的变化以及生物膜中的功能预测.
- 为了比较不同微生物组和抗体组数据库的性能.
主要方法:
- 使用河水复制的微观宇宙系统.
- 生物膜连续暴露于低于MIC水平 (1/10,1/50,1/100 MIC) 的西普罗夫洛克萨,菌素和氧基环素,持续八周.
- 使用显微镜和元基因组学进行结构功能分析.
主要成果:
- 低于MIC的抗生素暴露改变了生物膜结构,减少了细胞外聚合物质和自身型/原生生物质.
- 微生物多样性在最高的亚MIC下降 (1/10),而抗体多样性在1/50的亚MIC上达到顶峰.
- 确定了关键的抗微生物耐药性基因 (ARG) 和蛋白质细菌与氨基糖化物耐药性之间的联系.
结论:
- 长期的亚MIC抗生素暴露促进了抗生素耐药性的流行和河流生物膜中的传播.
- 观察到微生物社区结构和代谢功能的显著变化.
- 数据库选择和基准测试对于准确的ARG元基因组分析至关重要.
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