周围血液中的NK细胞携带来自固体癌细胞的瘤抗原
Mauricio Campos-Mora1, William Jacot2, Genevieve Garcin1
1IRMB, Univ Montpellier, INSERM, Montpellier, France.
Frontiers in immunology
|August 18, 2023
概括
在乳腺癌患者中,自然杀手 (NK) 细胞可以通过巨细胞化获得瘤抗原. 然而,很少有循环NK细胞表现出这些标记物,这表明从瘤微环境中退出有限.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
背景情况:
- 自然杀手 (NK) 细胞是关键的先天性免疫淋巴细胞,透瘤并消除癌细胞,影响患者的预后.
- 虽然NK细胞的透已知,但这些抗瘤NK细胞是否会离开瘤微环境仍然不清楚.
- 在血液癌症中,与白血病细胞相互作用的NK细胞表现出特定的标记物,如CD45异型共同表达和通过囊细胞分裂获得的瘤抗原.
研究的目的:
- 为了研究NK细胞通过囊细胞化通过固体瘤抗原的吸收.
- 分析乳腺癌 (BC) 患者和健康捐赠者 (HD) 的NK细胞子集,以确定潜在的抗瘤NK细胞群.
- 确定BC患者中循环NK细胞是否携带瘤抗原相互作用的标记物,类似于血液癌症中观察到的标记物.
主要方法:
- 在体外共同培养实验中,确认NK细胞对固体瘤抗原的输血细胞.
- 无监督的尺寸缩小技术 (FlowSOM) 用于分析来自BC患者和HD患者的血液样本中的NK细胞子集.
- 流细胞测量以确定NK细胞表型,包括CD45异型表达和瘤抗原的存在.
主要成果:
- 已证实NK细胞在体外对固体癌症抗原进行细胞化,其变异性取决于细胞和抗原.
- 在BC患者和HD患者之间,多个NK细胞群差异丰富,包括CD45RARO+CD107a+抗瘤NK子集.
- 在BC患者中,与瘤抗原结合的脱粒 NK 细胞增加,但它们的频率非常低,表明从瘤环境中几乎没有退出.
结论:
- 这项研究提供了第一份关于乳腺癌患者循环NK子集上固体瘤标记物的证据.
- 已确定的抗瘤NK细胞子集 (CD45RARO+CD107a+) 显示出作为生物标志物的潜力.
- 低频率的细胞化NK细胞表明,瘤的退出有限,可能会为乳腺癌的新疗法策略提供信息.
关键词:
CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45 CD45在 NK 细胞中,NK 细胞是 NK 细胞.乳腺癌 乳腺癌 乳腺癌这就是T-SNENE.输血细胞形成的过程更多相关视频
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