通过对最小扰乱的组织相关微生物群进行分析,通过分析结直肠癌相关细菌
Hironori Fukuoka1, Dieter M Tourlousse2, Akiko Ohashi2
1Department of Gastroenterological Surgery, Yokohama City University Hospital, Yokohama, Japan.
Frontiers in cellular and infection microbiology
|August 18, 2023
概括
这项研究分析了结肠癌 (CRC) 组织中的肠道细菌,没有肠道准备,发现与瘤相关的微生物高度局部化,并且在患者之间有很大差异. 进一步的研究将澄清CRC中的这些微生物差异.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 在瘤学瘤学.
背景情况:
- 肠道微生物群分析有助于检测结肠直肠癌 (CRC),但通常会受到肠道准备的影响.
- 了解CRC瘤的现场微生物群对于准确的诊断和治疗至关重要.
研究的目的:
- 在没有手术前肠道准备的患者的组织样本中研究CRC相关的粘膜细菌.
- 评估瘤相关微生物群的空间分布和患者间的变异性.
主要方法:
- 从11名未做好准备的CRC患者的瘤和相邻非癌性组织的高通量16SrRNA基因测序.
- 粘膜微生物群的厘米尺度分析.
- 短期液体培养和基因组组合的元基因组学.
主要成果:
- 观察到瘤内外微生物群组成的显著患者间变异.
- 瘤相关粘膜中的特定细菌类型的丰富性高度局部化,在几厘米内减少.
- 产生了100多个元基因组组装基因组,包括在瘤样本中丰富的细菌.
结论:
- 这项研究提供了关于CRC组织中大部分未被扰乱的粘膜微生物群的见解.
- 在瘤局部化微生物群中观察到的变异性凸显了在更大的队列中进一步调查的必要性.
- 研究结果表明,与结直肠癌相关的局部微生物特征.
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