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酶选择,优化和生产,以实现大规模的消费后聚乙烯二甲的生物降解

Ya-Hue Valerie Soong¹, Umer Abid¹, Allen C Chang²

¹Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, Massachusetts, USA.

Biotechnology journal

|August 18, 2023

概括

研究人员设计了酶,以有效地生物降解塑料聚乙烯二甲 (PET). 这项研究优化了酶生产和降解,以实现可扩展,经济的生物回收过程,为循环PET经济铺平了道路.

科学领域:

生物技术和合成生物学
环境科学与工程环境科学与工程
聚合物科学与工程聚合物科学与工程

背景情况:

聚乙烯二甲 (PET) 是一种广泛使用的塑料,因其化学稳定性而闻名于环境持久性.
酶工程和聚水解酶的发现为PET生物循环提供了有前途的途径.
开发有效和经济的方法来降解消费后的PET废物对于循环经济至关重要.

研究的目的:

为消费后废弃物PET开发一个大规模,高效,经济的生物降解工艺.
选择,优化和生产高产量的PET降解酶.
为了证明回收PET的高容量酶降解.

主要方法:

在*Escherichia coli*中优化和表达PETase,MHETase和叶枝堆肥化酶 (LCC^ICCG) 变体.
设计LCC^ICCG变体,其中包括用于分泌的pelB领导序列和用于增强PET表面结合的基底结合模块 (PBM).
开发一种食批次发酵工艺,用于生产高产酶 (高达1.2g L-1) 和随后的酶降解试验.

主要成果:

成功生产工程LCC^ICCG变种和其他PET降解酶的高产量.
确定PelB-LCC作为PET降解最有效的酶变体.
使用PelB-LCC酶在受控反应器系统中证明200克L-1回收PET的高效降解.

结论:

工程酶,特别是PelB-LCC,显示出PET生物循环的巨大潜力.
开发的高产量生产和降解方法是经济和可扩展的生物回收过程的关键.
这项研究有助于通过可持续的塑料废物管理建立循环 PET 经济.

关键词:

酸酶 (PETase) 是一种生物降解生物降解叶子-树枝堆肥库蒂纳酶的使用.聚乙烯甲酸) 是一种聚乙烯甲酸.乙烯基乙烯酸 (乙烯基乙烯酸) 是一种

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