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相关概念视频

Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

5.0K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
5.0K
Insertion of Multi-pass Transmembrane Proteins in the RER01:29

Insertion of Multi-pass Transmembrane Proteins in the RER

8.1K
The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
8.1K
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.7K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.7K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

4.6K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.6K
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

5.3K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
5.3K
Primary Active Transport01:29

Primary Active Transport

10.3K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
10.3K

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相关实验视频

Updated: Jul 19, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

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TMKit:一种用于对跨膜蛋白进行计算分析的 Python 接口.

Jianfeng Sun1, Arulsamy Kulandaisamy2, Jinlong Ru3

  • 1Nuffield Department of Orthopedics, Rheumatology, and Musculoskeletal Sciences, Botnar Research Centre, University of Oxford, Headington, Oxford OX3 7LD, UK.

Briefings in bioinformatics
|August 18, 2023
PubMed
概括
此摘要是机器生成的。

研究人员现在可以使用TMKit,一个新的开源Python工具包,更有效地分析跨膜蛋白. TMKit提供了用于序列和结构分析的专用计算工具,解决了生物信息学中的关键差距.

关键词:
生物信息学是一种生物信息学.功能提取 特性提取蛋白质相互作用接口.序列分析分析的序列分析.结构生物学结构生物学跨膜蛋白质是一种跨膜蛋白质.

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A Protocol for Computer-Based Protein Structure and Function Prediction
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A Protocol for Computer-Based Protein Structure and Function Prediction

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Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
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Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

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相关实验视频

Last Updated: Jul 19, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.3K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

68.7K
Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

91

科学领域:

  • 生物化学和分子生物学
  • 计算生物学 计算生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 跨膜蛋白对于信号传导和通信等细胞功能至关重要.
  • 现有的计算工具缺乏跨膜蛋白质分析的专业化,造成了研究缺口.

研究的目的:

  • 介绍TMKit,一个开源的Python编程界面,用于专门的跨膜蛋白数据分析.
  • 为数据库纠纷,特征工程和跨膜蛋白质可视化提供全面的工具包.

主要方法:

  • 开发TMKit,一个模块化和可扩展的Python接口.
  • 集成seqNetRR,用于残留连接分析的高性能计算库.
  • 实现用于突变,域和拓特征工程的工具以及蛋白质-蛋白质相互作用接口的可视化.

主要成果:

  • TMKit为各种跨膜蛋白质分析提供了一个统一的平台.
  • seqNetRR库能够快速分配基于关联矩阵的特征.
  • 该工具包促进了跨膜蛋白数据的高效处理和分析.

结论:

  • TMKit解决了跨膜蛋白研究中专用计算工具的需求.
  • 这个开源工具包增强了对跨膜蛋白序列和结构的研究.
  • TMKit是公开提供的,以支持研究界.