通过使用4-APEBA的组织化学衍生物进行质谱成像来扩大植物化合物的覆盖范围
Kevin J Zemaitis1, Vivian S Lin1, Amir H Ahkami1
1Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Analytical chemistry
|August 18, 2023
概括
这项研究引入了一种新的组织化学衍生物化 (OTCD) 工作流程,使用4-APEBA显著增强空间代谢. 该方法改善了难以捉摸的植物化合物和植物激素的检测,推动了植物科学研究.
科学领域:
- 植物科学 植物科学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 细胞尺度上的代谢学提出了重大的分析挑战.
- 矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 提供空间代谢学能力,但分子覆盖范围有限.
研究的目的:
- 开发一个改进的组织化学衍生物化 (OTCD) 工作流程,以增强空间代谢.
- 扩大难以捉摸的植物化合物的识别,并使植物样本中的空间荷尔蒙学成为可能.
主要方法:
- 使用4-APEBA.的组织化学衍生 (OTCD) 工作流程的开发.
- 应用MALDI-MSI在连续植物组织段上用于代谢物概况.
- 用于数据处理和代谢物注释的生物信息分析.
主要成果:
- 4-APEBA OTCD 工作流使大约 280 种代谢物的注释成为可能,与传统的负离子模式 MALDI-MSI 只有 10% 的重叠.
- 4-APEBA对碳基具有广泛的特异性,背景噪声低,并引入同位素以提高数据可靠性.
- 该方法成功地在树和大豆根组织中分析了几种植物激素,包括碳酸盐和化物.
结论:
- 开发的OTCD工作流显著扩大了植物空间代谢和空间荷尔蒙学的范围.
- 4-APEBA是一种卓越的衍生剂,用于检测具有挑战性的代谢物和植物激素.
- 这种方法通过改进的空间概况,提高了对植物应激反应和细胞通信的理解.
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