循环POLA2介导的miR-138-5p/SEMA4C轴影响结肠癌细胞活动
YanDong Huang1, QingYang Bai2, HongBo Yu3
1Department of Oncology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou City, Inner Mongolia Autonomous Region, 014017, China. 18504720110@163.com.
Acta biochimica Polonica
|August 18, 2023
概括
循环RNAPOLA2 (circ-POLA2) 通过调节miR-138-5p/SEMA4C通路来促进结肠癌 (CC) 的进展. 准这一轴为结肠癌提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 结肠癌 (CC) 仍然是一个重大的全球健康挑战.
- 了解驱动CC进展的分子机制对于开发有效疗法至关重要.
研究的目的:
- 阐明圆形RNAPOLA2 (circ-POLA2) 在结肠癌中的作用和机制.
- 在CC中调查涉及circ-POLA2,miR-138-5p和SEMA4C的监管轴.
主要方法:
- 在CC组织和细胞中对circ-POLA2,miR-138-5p和SEMA4C表达的定量分析.
- 评估circ-POLA2,miR-138-5p和SEMA4C对癌细胞增殖,迁移,入侵和亡的影响.
- 探索circ-POLA2,miR-138-5p和SEMA4C之间的反循环.
主要成果:
- 在结肠癌中,Circ-POLA2和SEMA4C被发现是上调的,而miR-138-5p则是下调的.
- 通过海绵化miR-138-5p,Circ-POLA2被证明可以通过上调SEMA4C.
- 抑制circ-POLA2抑制的CC细胞活动,通过抑制miR-138-5p或SEMA4C过度表达而逆转.
结论:
- 通过调节miR-138-5p/SEMA4C轴,Circ-POLA2在结肠癌中充当瘤基因.
- 这种circ-POLA2/miR-138-5p/SEMA4C通路代表了结肠癌治疗的潜在治疗标.
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