细胞外聚合物中有线状蓝藻和疏水性蛋白质在部分化过程中促进藻类-细菌聚合
Mei Zhi1, Yiying Zhao2, Xinyu Zeng2
1College of Resources and Environment, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Rural Cleaner Production, Chongqing 400715, China.
International journal of biological macromolecules
|August 18, 2023
概括
短固体保留时间 (SRT) 促进藻类-细菌聚合物在废水处理中沉积. 纤维状蓝藻和特定的细胞外聚合物物质 (EPS) 结构增强聚合物的形成和功能.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废水处理工程 废水处理工程
背景情况:
- 在藻类-细菌共生废水处理中,有效的生物质分离依赖于藻类-细菌聚合物的沉性能.
- 了解影响聚合物形成和沉积的因素对于优化废水处理过程至关重要.
研究的目的:
- 研究不同固体保留时间 (SRT) 对细胞外聚合物物质 (EPS) 组成,微生物特征和藻类-细菌聚合物的功能基因的影响.
- 阐明海藻-细菌聚合背后的机制,以及在光测序生物反应器 (PSBR) 中在部分化条件下的沉积性能.
主要方法:
- 藻类-细菌聚合物在三个不同的SRT (10,20和40天) 中在PSBR中培养.
- 分析了EPS的组成,微生物社区结构 (包括藻类和细菌) 和功能基因表达.
- 总颗粒大小和沉速度被测量以评估性能.
主要成果:
- SRTs显著影响了转化和藻类-细菌聚合物的形成/形态.
- 最短的SRT (10d) 导致化积累最高,颗粒尺寸最大 (~1.54毫米),沉性能最佳.
- 在10d SRT时,聚合物显示了丰富的线状蓝藻细菌,高的叶绿素a/b比率,低的EPS含量和高的蛋白质与多糖合物比率,表明EPS结构较松散.
结论:
- 纤维状蓝藻和特定的EPS结构,以更高的蛋白质含量和更松散的结构为特征,是促进藻类-细菌聚合和提高沉性能的关键因素.
- 与氨基酸代谢相关的功能基因在最佳SRT时高度表达,这表明它们在总稳定中的作用.
- 这项研究揭示了光颗粒结构和功能背后的生物机制,为优化共生废水处理系统提供了见解.
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