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通过使用高效的微生物纤维素从水黄废物中生产的纤维素生物转化生产可发酵葡萄糖

Manikant Tripathi¹, Basant Lal², Asad Syed³

¹Biotechnology Program, Dr. Rammanohar Lohia Avadh University, Ayodhya 224001, Uttar Pradesh, India.

International journal of biological macromolecules

|August 18, 2023

概括

这项研究通过固态发酵增强了使用水黄废物的细胞酶生产. 优化的条件产生了显著的酶活性,使生物质中的葡萄糖能够有效地从生物质中释放出来,用于生物炼油应用.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
生物质转换生物质转换

背景情况:

细胞酶对于生物燃料生产等工业应用至关重要,但其生产成本昂贵.
开发使用丰富,低成本基质的成本高效的纤维素生产方法对于生物炼油厂至关重要.

研究的目的:

通过固态发酵 (SSF) 提高细胞酶的生产和活性,利用水黄 (WH) 废物作为低成本基质.
优化工艺参数 (温度,pH值) 以提高细胞酶产量和活性.
为了评估产生的细胞酶在化WH生物质以释放葡萄糖中的有效性.

主要方法:

水 (叶子和茎) 的固态发酵 (SSF) 用于产生纤维素.
研究过程参数,包括温度和pH值,以获得最佳的酶生产和活性.
使用优化的原始细胞酶酶对水叶的酶性水解.

主要成果:

优化的SSF条件 (10g原料,第5天) 产生了显著的酶活性:22 IU/gds FP,92 IU/gds BGL和111 IU/gds EG.
细胞酶生产的最佳温度是40°C,而酶活性的最佳反应温度是50-55°C.
生产的最佳pH值为5.5;该酶在55°C和pH值5.0下在3.5小时内表现出热稳定性. 在WH叶的水解中,在24小时内释放24.34g/L的葡萄糖.

结论:

水黄废物是一种可行的,低成本的基质,可以通过SSF增强细胞酶的生产.
优化过程参数显著提高了酶产量和活性.
开发的细胞酶显示了高效生物质水解的潜力,支持生物炼油应用.

关键词:

生物燃料是一种生物燃料.细胞质酶是一种细胞质酶.纤维素纤维素是一种纤维素.葡萄糖是一种葡萄糖.水花水花水花

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