在快速检测病原体中应用CRISPR/Cas12a
Yiheng Wang1, Tianmu Yang1, Guifang Liu1
1Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
概括
在CRISPR-Cas12a系统提供快速和敏感的检测致病性细菌. 本综述探讨了其机制以及开发改进的诊断平台的进展.
科学领域:
- 分子生物学分子生物学
- 生物技术是生物技术.
- 遗传学 是一个遗传学.
背景情况:
- 聚类定期间隔的短平行体重复 (CRISPR) 和 Cas 蛋白质形成了有效的基因编辑工具.
- 克里斯普尔-Cas12a系统利用crRNA进行DNA向,并表现出跨裂变的活性.
- 克里斯普技术为生物目标提供快速检测和高灵敏度.
研究的目的:
- 审查CRISPR-Cas12a在快速检测中的机制.
- 要总结CRISPR-Cas12a在检测病原细菌方面的进展.
- 为开发先进的检测平台提供基础.
主要方法:
- 审查关于CRISPR-Cas12a的现有文献.
- 在基因检测中分析CRISPR-Cas12a机制.
- 整合CRISPR-Cas12a与分子生物学技术用于病原体识别.
主要成果:
- 在放大后,CRISPR-Cas12a可以有效地检测致病基因.
- 该系统的跨裂变活动,加上光探针,使敏感的诊断成为可能.
- 克里斯普尔-Cas12a可以更快,更精确地诊断细菌病原体.
结论:
- CRISPR-Cas12a是一个强大的工具,用于快速和灵敏地检测致病细菌.
- 将CRISPR-Cas12a与各种分子技术相结合,可以提高诊断能力.
- 进一步开发CRISPR-Cas12a平台有望提供更有效的病原体检测解决方案.
相关概念视频
01:28CRISPR/Cas9 Genome Editing
54
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
54
02:53CRISPR and crRNAs
17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
01:59CRISPR
52.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.3K