通过同位素稀释质谱法量化改性核酸和核酸的量化
Juan M Marchante-Gayón1, Jesús Nicolás Carcelén1, Helí Potes Rodríguez1
1Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Oviedo, Spain.
Mass spectrometry reviews
|August 19, 2023
概括
本综述详细介绍了使用染色学和质谱学量化DNA甲基化修饰的进展,包括5-甲基和其氧化产物. 这些方法对于了解癌症等疾病至关重要.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 表观遗传修饰,特别是DNA甲基化 (5-甲基-2'-脱氧丁或5-mdC),与包括癌症在内的各种疾病有关.
- 5-mdC的氧化产生了5-(基甲基) -2'-脱氧丁,5-甲基-2'-脱氧丁和5-碳基-2'-脱氧丁,增加了表观遗传调节的复杂性.
研究的目的:
- 审查最近 (过去20年) 修改DNA细胞因子量化方面的进展.
- 为突出应用同位素稀释技术与染色学质谱相结合,以实现精确的测量.
- 讨论染色学方法和衍生策略,以进行增强分析.
主要方法:
- 液体染色学与双重质谱学 (LC-MS/MS) 结合,是量化修改DNA细胞因子的主要技术.
- 用同位素标记的类似物进行同位素稀释,用于精确量化.
- 逆相和水友相互作用液态染色学 (RPLC和HILIC) 是常用的,与衍生方法一起用于提高分辨率和电离.
主要成果:
- 在生物样本中量化甲基化DNA细胞因子及其氧化产物方面取得了重大进展.
- 同位素标记化合物作为准确可靠测量的内部标准.
- 二维色谱也被用于复杂的样本分析.
结论:
- 精确量化DNA修饰对于理解它们在生物过程和疾病中的作用至关重要.
- 结合同位素稀释和先进的色谱技术,LC-MS/MS为表观遗传学研究提供了强大的工具.
- 这些分析进步对生物和临床应用有重大影响,特别是在瘤学中.
关键词:
气体和液体色谱学 气体和液体色谱学同位素稀释的同位素稀释质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量甲基化和改性核酸和核酸.更多相关视频
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
9.5K
12:49Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
11.7K
相关概念视频
Mass Spectrometry: Isotope Effect
2.2K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
2.2K
Mass Spectrum: Interpretation
1.3K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
To...
1.3K
High-Resolution Mass Spectrometry (HRMS)
1.4K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.4K
Mass Spectrometry: Overview
5.4K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.4K
