在性结肠炎模型中,Ganoderma lucidum β-d-glucans具有不同分子量的抗炎和肠道微生物群调节特性
Liping Liu1, Jie Feng1, Siqi Jiang1
1Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
甘白 (Ganoderma lucidum) 的β-D-葡萄糖通过调节炎症和肠道微生物群,对性结肠炎 (UC) 产生治疗作用. 通过降解降低分子量可以增强这些抗炎活性,这表明UC治疗的潜力.
科学领域:
- 菌类学 菌类学是指菌类学.
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,治疗选择有限.
- 光 (G. lucidum) 的β-D-葡萄糖因其免疫调节特性而闻名.
- 分子重量 (Mw) 对G. lucidumβ-D-glucans在UC治疗疗效的影响尚未完全理解.
研究的目的:
- 在小鼠模型中研究G. lucidum beta-D-glucans具有不同的Mws对硫酸 (DSS) 诱导的性结肠炎 (UC) 的治疗作用.
- 阐明潜在的机制,包括炎症性细胞因子的调节,肠道免疫,肠道微生物群和短链脂肪酸 (SCFA).
主要方法:
- 用不同的Mw (GLPS,GLPN,GLPW) 制备和分离G. lucidumβ-D-葡萄糖.
- 在小鼠中使用DSS诱导UC.
- 服用β-D-葡萄糖分数和评估UC症状.
- 对炎症性细胞因子水平,肠道免疫标记物,肠道微生物群组成和SCFA概况的分析.
主要成果:
- 所有测试的G. lucidumβ-D-葡萄糖 (GLPS,GLPN,GLPW) 在减少UC症状方面都显示出显著的治疗效果.
- 与中等 (GLPN) 和高 (GLPS) Mw分数相比,微波辅助降解分数 (GLPW) 具有较低的Mw,显示出与中等 (GLPN) 和高 (GLPS) Mw分数相比的可比或优异的抗炎症和SCFA-代谢调节作用.
- 与GLPN相比,GLPW在调节肠道微生物群组成方面表现出更高的有效性.
结论:
- 降低G. lucidumβ-D-葡萄糖的Mw的物理降解可以增强它们的抗炎活性和UC的治疗潜力.
- 降解的G. lucidumβ-D-葡萄糖为UC的预防和治疗提供了一个有希望的途径,由对肠道微生物群和SCFA调节的机械洞察支持.
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