在现场形成宏的生物混合水凝,用于核脉细胞的输送
Amanda J Brissenden1, Brian G Amsden1
1Department of Chemical Engineering, Queen's University, Kingston, ON, Canada K7L 3N6.
Acta biomaterialia
|August 20, 2023
概括
这项研究开发了一种用于核脉 (NP) 再生的新型水凝,显示出治疗退行性磁盘疾病的前景. 水凝支持NP细胞生长和矩阵沉积,模仿本地组织特性.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 退行性椎间盘疾病是慢性疼痛的主要原因,通常始于核脉 (NP).
- 目前的治疗方法缺乏有效的NP组织再生策略.
- 在支持性水凝中进行最小侵入性细胞输送提供了一个有前途的治疗方法.
研究的目的:
- 为了合成和表征一种新的生物混合型triblock共聚合物水凝,用于NP细胞的递送和再生.
- 评估水凝支持NP细胞活力,增殖和细胞外基质沉积的能力.
- 评估水凝的机械和水性质与原生NP组织相比.
主要方法:
- 合成一个ABA二甲基酸三块化共聚合物,其较低的临界溶液温度低于37°C,以及一个MMP可降解的中心块.
- 通过氧化还原启动的交叉连接形成宏的,NP细胞载的水凝,有或没有硫酸化.
- 评估水凝的物理化学性质,机械弹性和NP细胞在低氧培养中28天的行为.
主要成果:
- 合成的水凝表现出与原生人类NP组织相比的水和机械性能.
- 在28天的时间里,水凝成功地支持了NP细胞的附着,生长和均分布.
- 水凝中的NP细胞沉积了II型原体和硫酸糖氨基甘油,表明了矩阵再生.
结论:
- 开发出来的宏性水凝是NP细胞递送和再生的可行支架.
- 水凝配方支持NP细胞功能和矩阵生产,显示治疗退行性磁盘疾病的潜力.
- 需要进一步研究这种水凝作为NP组织再生的细胞输送载体.
相关概念视频
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