相关实验视频

Updated: Jul 18, 2025

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

Published on: June 3, 2021

发素在小鼠中的毒动力学和组织分布

Chang Ho Lim¹, Im-Sook Song², Jihoon Lee²

¹College of Pharmacy and BK21 Four-sponsored Advanced Program for SmartPharma Leaders, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|August 20, 2023

概括

中的毒素法洛伊丁 (Phalloidin) 具有可预测的药理动力学和较低的口服吸收. 由于特定的输送器参与,肝脏,脏和肠道是主要目标.

科学领域:

药理学药理学是指药理学的学科.
毒理学毒理学毒理学
药物代谢和药理动力学

背景情况:

已知Phalloidin是来自Amanita的循环七分,通过结合F-actin而引起肝毒性.
需要进一步的研究来了解法洛伊丁的毒动力学,组织分布和潜在机制.

研究的目的:

在静脉和口服服后调查法洛丁的毒动学和组织分布.
阐明有助于法洛伊丁分布的机制,特别是其在肝脏和脏中的吸收.

主要方法:

用法洛伊丁静脉注射 (0.2-0.8毫克/公斤) 和口服 (2-10毫克/公斤) 来确定药理学参数.
使用Caco-2细胞单层评估了法洛伊丁的吸收透性.
在度-时间曲线 (AUC) 下测量的组织-血面积为各种器官的比率.

主要成果:

在血度曲线 (AUC) 下的法洛伊丁呈现的剂量比例面积增加.
观察到剂量独立,分布和清除量低,口服生物利用率低 (2.4%-3.3%).
在肝脏 (IV) 和肠道 (口腔) 中发现了高组织-血AUC比率,在脏中也发现了显著水平,这表明活跃的运输参与.

结论:

洛伊丁显示可预测的药理动力学与有限的口服吸收.
肝脏,脏和肠道是法洛伊丁积累的关键目标器官.
肝脏和脏的有机离子载体 (OATP1B1,OATP1B3,OAT3) 可能会调解法洛因的分布到肝脏和脏.

关键词:

活动运输活动运输口服吸收口服吸收洛伊丁是一种化物.组织分布组织分布毒动力学毒动力学毒动力学

更多相关视频

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

Published on: January 7, 2015

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Published on: February 2, 2018

相关概念视频

Drug Distribution: Tissue Binding

Drug Distribution: Tissue Binding

Upon entering the systemic circulation, drugs can distribute into the interstitial and intracellular fluid of various tissue cells. This distribution is facilitated by the binding of drugs to different cellular components within tissues, which may lead to drug accumulation in specific areas. Drugs bound to tissue components serve as reservoirs that release free drugs back into the system, prolonging the drug's overall action. However, this accumulation can also result in local toxicity.
For...