相关实验视频

Updated: Jul 18, 2025

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Published on: May 16, 2017

经验依赖的Tip60核细胞质运输是由其NLS/NES序列调节的,用于控制神经可塑性基因

Ellen M Armour¹, Christina M Thomas¹, Gabrielle Greco¹

¹Department of Biology, Drexel University, Philadelphia, PA, United States of America.

Molecular and cellular neurosciences

|August 20, 2023

概括

神经刺激驱动Tip60基因组酸转移酶核导入用于记忆和阿尔茨海默氏症中的基因调节.

科学领域:

神经科学是一个神经科学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

核细胞质运输 (NCT) 对神经元功能至关重要,调节可塑性基因对记忆和认知过程至关重要.
经验依赖的 (ED) 神经可塑性,由素乙化 (HA) 驱动,与阿尔茨海默氏症 (AD) 等神经退行性疾病有关.
通过酸转移酶 (HATs) 响应细胞外线来控制基因活性的体内机制在很大程度上是未知的.

研究的目的:

调查Tip60 HAT亚细胞局部化和NCT在神经元活动依赖基因控制中的作用.
通过使用Drosophila体 (MB) 作为学习和记忆的模型,在体内检查Tip60 HAT的作用.
在AD的Drosophila模型中探索Tip60NCT的潜在破坏.

主要方法:

免疫组织化学 (IHC) 评估Tip60 HAT在Drosophila大脑中的定位在正常和刺激条件下.
通道激活的遗传诱导和暴露在神经元刺激的自然阳性ED条件下.
核定位信号 (NLS) 和核出口信号 (NES) 序列的突变发生在Drosophila Tip60.

主要成果:

通过遗传或ED途径的神经元刺激,诱导了Tip60 HAT核导入和基因表达.
在Drosophila AD模型中,Tip60 HAT水平在细胞核和细胞质中显著下降.
功能性突变发生证实了NLS和NES序列对于Tip60 HAT适当的亚细胞定位的要求.

结论:

神经元刺激通过其NLS和NES触发Tip60NCT,促进活动依赖的神经可塑性基因转录.
破坏Tip60 NCT可能会导致阿尔茨海默氏症中观察到的病理.
Tip60 HAT的动态亚细胞局部化是将神经元活动与表观遗传基因调节联系起来的关键机制.

关键词:

活动依赖基因控制阿尔茨海默病 (AD) 是一种疾病.神经可塑性基因的神经可塑性基因核定位序列 (NLS) 是指一个核定位序列.核细胞质运输 (NCT) 是一个过程.Tip60 基因组乙转移酶 (HAT) 的作用

更多相关视频

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

相关概念视频

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Nuclear Export

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...

Neuroplasticity

Neuroplasticity

Neuroplasticity reflects the brain's remarkable capacity to adapt and evolve, responding dynamically to learning, experiences, or injury by reorganizing its neural circuitry. This reorganization involves creating new neural connections and refining old ones through a series of biological processes that contribute to the brain's lifelong development and adaptability.