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蒂莫基诺因对NRK-52e细胞内质内网膜 (ER) 应激的作用
- Muhammed Celebi1, - Semiha Dede2, - Ayşe Usta3
1Van Yuzuncu Yil University, Institute of Health Sciences, Van, Turkiye.
Pakistan journal of pharmaceutical sciences
|August 21, 2023
概括
蒂莫金 (TQ) 影响细胞内核细胞网膜 (ER) 压力基因. TQ显著改变ER压力基因表达,特别是在增殖度,表明对健康细胞的潜在影响.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 细胞内膜网膜 (ER) 管理蛋白质折叠和转移.
- 当未折叠或错误折叠的蛋白质在ER光线中积聚时,ER压力发生.
- 尼日利亚的活性成分,Thymoquinone (TQ),表现出各种有益的影响.
研究的目的:
- 为了研究提摩 (TQ) 对ER应激的体外作用.
- 分析TQ对NRK-52E细胞在不同度和时间点的ER压力基因表达的影响.
主要方法:
- 使用NRK-52E细胞进行体外研究.
- 在增殖性 (Tp) 和有毒性 (TQIC50) 度下使用提摩 (TQ) 治疗.
- 在24小时和48小时内分析ER应激通路基因表达 (ATF4,ATF6,BIP,CHOP,IRE1,XBP1,PERK).
主要成果:
- 在增殖度 (Tp) 时,大多数ER压力基因 (CHOP和XBP1除外) 在24小时增加,BIP在48小时增加.
- 在有毒度 (TQIC50) 时,CHOP和XBP1在24小时后增加,而CHOP和ATF4在48小时后增加.
- 观察到ER压力基因表达的显著变化,取决于TQ度和潜伏时间.
结论:
- 蒂莫金 (TQ) 在细胞中显著改变ER应激基因表达.
- TQ对ER压力的影响是依赖于度和时间的.
- 对TQ对健康细胞影响机制的进一步研究是有必要的.
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