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从预处理的米中生产生物乙醇:对发酵产量,结构特征和乙醇分析的影响

Reema Ningthoujam¹, Pankaj Jangid¹, Virendra Kumar Yadav²

¹Department of Biosciences, School of Liberal Arts and Sciences (SLAS), Mody University of Science and Technology, Lakshmangarh, Rajasthan, India.

Frontiers in bioengineering and biotechnology

|August 21, 2023

概括

草生物质的性预处理显著提高了乙醇的生产. 这种具有成本效益的方法提高了糖产量,并使用Saccharomyces cerevisiae实现了21.45%的高最终乙醇度.

科学领域:

生物质转化和生物能源
生物化学工程生物化学工程
可持续化学可持续化学

背景情况:

在可持续的乙醇生产中,有效地将纤维素生物质转化为可发酵糖至关重要.
生物质预处理是一个重要的成本因素,需要具有成本效益和高效率的方法.
米 (RS) 是一个丰富的纤维素资源,具有生产生物燃料的潜力.

研究的目的:

对草进行性预处理以提高乙醇生产的有效性进行研究.
分析处理后大米的结构和形态变化.
为了比较使用预处理和本地大米的酶解水解和发酵效率.

主要方法:

用4%的NaOH预处理大米,浸泡4小时,自制30分钟.
通过富里埃变换红外光谱 (FTIR),X射线衍射 (XRD) 和扫描电子显微镜 (SEM) 进行结构和形态分析.
使用商业酶和微生物水解的酶化水解,然后用Saccharomyces cerevisiae发酵.

主要成果:

FTIR证实了显著的木质素去除. XRD显示结晶体大小增加. SEM揭示了扭曲的结构和增加的多孔性.
在优化条件下,使用商业酶降解糖的产量为0.62 g/L.
在特定的发酵参数下,预处理的RS的最高乙醇度达到21.45%,比未处理的RS的3.67%大幅增加.

结论:

性预处理有效地改变了米的结构,提高了其适用于酶解水解和发酵的适度.
与未经处理的生物质相比,优化的预处理和发酵过程显著提高了乙醇产量.
这项研究证明了一种成本效益高效的方法,用于将纤维素基生物质转化为乙醇.

关键词:

生物转化生物转化生物燃料生物燃料是生物燃料.生物质的生物质是生物质.发酵发酵发酵发酵预处理预处理米草草草

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