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石墨烯氧化物加快了TGFβ介导的上皮细胞-介质细胞过渡,并刺激了羊上皮细胞的亲炎性免疫反应

Adrian Cerverò-Varona¹, Angelo Canciello¹, Alessia Peserico¹

¹Department of Biosciences and Agro-Food and Environmental Technologies, University of Teramo,64100, Teramo, Italy.

Materials today. Bio

|August 21, 2023

概括

胚胎上皮质干细胞 (AECs) 上的石墨烯氧化物 (GO) 涂层加快了它们向迁移性介质细胞状态的过渡. 这增强了免疫细胞的激活,促进了组织再生战略.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
免疫学免疫学免疫学

背景情况:

生物材料对于组织工程中的免疫再生策略至关重要.
石墨烯氧化物 (GO) 影响干细胞行为和免疫反应,但其对羊水表皮干细胞 (AECs) 的影响尚不清楚.
由于其可塑性和免疫调节性质,AEC具有临床兴趣.

研究的目的:

调查GO对AECs的生物模拟效应.
探索GO在调节再生疗法的AEC行为方面的潜力.

主要方法:

在GO功能化的玻璃盖片上培养AEC.
分析表皮-半机体过渡 (EMT) 过程.
研究TGFβ1-SMAD2/3信号通路.
评估细胞因子分泌和免疫细胞相互作用的变化.

主要成果:

在AEC中,GO涂层通过TGFβ1-SMAD2/3信号传输诱导和加速EMT.
中酶体AEC获得迁移能力并改变细胞因子分泌.
暴露于GO的AEC增加了促炎性白内素,增强了巨细胞的激活.
在GO上的AEC稍微降低了对外围血液单核细胞增殖的抑制.

结论:

具有GO功能的表面可以为再生应用量身定制AEC行为.
GO促进了AEC的跨差异化,增强了它们的免疫调节作用.
这种方法可以为管理炎症和促进再生创造有益的微环境.

关键词:

集体迁移集体迁移皮质 - 介质细胞过渡 (EMT)石墨烯氧化物 (GO) 是一种免疫调节是一种免疫调节.炎症炎症是一种炎症.

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TGF - β Signaling Pathway

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The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...