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脂肪衍生干细胞在对齐的原I水凝中的神经再生行为
Mackenzie Lewis1, Gabriel David1, Danielle Jacobs1
1Department of Biomedical Engineering; University of Arkansas, Fayetteville, AR, USA.
Materials today. Bio
|August 21, 2023
概括
工程化原体支架与对齐的纤维和干细胞促进神经再生. 这种创新方法增强了血管生成因子和神经细胞外生长,用于外围神经的修复.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 周围神经受伤是一个重大的临床挑战,目前的治疗方法,如自体移植和合成导管具有局限性.
- 现有的制造脚手架经常与细胞均分布和指导神经生长作斗争,阻碍了最佳修复.
- 模仿本地神经环境对于有效的外周神经再生至关重要.
研究的目的:
- 开发和评估对齐的原I水凝支架与封装的脂肪衍生介质干细胞 (ASCs) 进行外周神经修复.
- 评估支架特性对细胞活力,机械支和神经再生潜力的影响.
- 为了研究ASCs在对齐的纤维支架中的神经再生能力.
主要方法:
- 使用定制设备制造配线纤维的原I水凝.
- 在水凝支架内封装脂肪衍生的介质干细胞 (ASCs).
- 评估细胞活力,机械性质 (Young的模量) 和*体外*神经再生结果,包括神经元外生和因子分泌.
主要成果:
- 在3毫克/毫升的原体凝 (84.8±7.3%) 与6毫克/毫升的凝 (76.7±9.5%) 相比,观察到最佳的细胞活力.
- 3毫克/毫升的凝呈现了6.5 ± 0.8 kPa的扬氏模量,与支持施万细胞迁移的7.45 kPa值密切匹配.
- *在体外* 伸展支架增强了血管生成因子分泌,ECM沉积,纤维对齐,以及沿纤维轴的背部根 (DRG) 神经元外生.
结论:
- 对齐的原I水凝支架与封装的ASC显示出对外围神经修复的希望.
- 脚手架的设计,包括纤维对齐和原度,显著影响细胞活力和再生潜力.
- 该平台提供了一个*体外*模型,用于评估神经再生策略和设计下一代神经修复支架.
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