开发一种检测多液中 lipoarabinomannan 的方法,并评估其对结核性多溢液的诊断疗效
Lijun Peng1, Lingshan Dai1, Mingzhi Zhu1
1Clinical Laboratory Center, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Heliyon
|August 21, 2023
概括
诊断结核肺流 (TPE) 是很困难的. 一个新的多液脂氨酸 (LAM) 测试显示出有希望的结果,特别是当与腺脱氨酶 (ADA) 测试相结合时,改善了TPE检测率.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 传染病 传染病 传染病
- 呼吸系统药物 呼吸系统药物
背景情况:
- 结核性流 (TPE) 诊断是具有挑战性的,因为传统方法的局限性.
- 准确有效的TPE诊断工具对于及时治疗至关重要.
研究的目的:
- 评估肺液 (PF) 的诊断性能的利波阿拉比诺南 (LAM) 结核性肺溢液 (TPE).
- 评估新建立的LAM-PF测定在诊断TPE时的有用性.
主要方法:
- 使用LEDBIO AIMLAM套件开发了PF LAM (LAM-PF) 的诊断方法.
- 在162名怀疑TPE的HIV阴性患者中评估了LAM-PF测定.
主要成果:
- LAM-PF测定显示出良好的线性和恢复,检测极限为2.90 pg/mL.
- 在切断值为5.33 pg/mL时,LAM-PF对确定的TPE显示了47.7%的灵敏度和100.0%的特异性.
- 对LAM和腺脱氨酶 (ADA) 的联合测试将TPE检测灵敏度提高到68.0%.
结论:
- 已建立的LAM-PF方法为TPE提供了有利的诊断性能,特别是在可能的TPE病例中.
- 将LAM和ADA测试结合在多液中显著提高了TPE的诊断效率.
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