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发现deoxyandrographolide及其通过向HDAC1对血管衰老的新效应

Zhongxiao Lin^1,2, Hao He¹, Yu Xian¹

¹State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy Macau University of Science and Technology Macau China.

|August 21, 2023

概括

来自富士的deoxyandrographolide (DA) 通过增强Histone deacetylase 1 (HDAC1) 以改善染色体稳定性,有效地治疗血管衰老 (VS). 这种化合物显示出作为治疗年龄相关血管疾病的新疗法具有前途.

科学领域:

药理学药理学是指药理学的学科.
老年学是一门学科.
分子生物学分子生物学

背景情况:

富士 (Aconitum carmichaelii) 是一种传统的中医药,具有很长的使用历史.
福齐的特定成分及其治疗血管衰老 (VS) 的机制尚不清楚.
血管衰老有助于各种与年龄有关的疾病.

研究的目的:

用网络药理学识别Fuzi中的活性化合物,用于治疗VS.
阐明鉴定到的化合物对抗VS的分子机制.
验证候选化合物对VS的治疗潜力.

主要方法:

网络药理学被用来预测潜在的活性化合物和标.
进行了体外和体内共同培养的研究,以评估deoxyandrographolide (DA) 对衰老生物标志物的影响.
虚拟查,生物层干涉测量,CRISPR/Cas9基因编辑和RNA测序被用来验证目标和机制.

主要成果:

脱氧andrographolide (DA) 被确定为富士的关键化合物,具有抗衰老特性.
DA显著抑制了诸如p16,p21,γH2A.X和p53这样的衰老生物标志物.
通过抑制其无化,DA增强了Histone deacetylase 1 (HDAC1) 的表达,这反过来抑制了H3K4me3并改善了染色体的稳定性.

结论:

作为血管衰老 (VS) 的新型治疗剂,DA显示出显著的潜力.
该机制涉及DA介导的HDAC1增强,从而改善染色体稳定性.
这项研究为开发针对VS和相关血管衰老疾病的DA治疗提供了基础.

关键词:

富士人富士人富士人在 HDAC1 中,HDAC1 是脱氧andrographolide 的使用情况.这是表观遗传学.网络药理学网络药理学

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