在巨细胞动脉炎和多肌痛类风湿症中,循环抗原呈现细胞的异常表型
Rosanne D Reitsema1,2, Bernd-Cornèl Hesselink1, Wayel H Abdulahad1,3
1Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
Frontiers in immunology
|August 21, 2023
概括
在巨细胞性关节炎 (GCA) 和多肌痛性风湿性关节炎 (PMR) 中,循环的非经典单细胞在诊断时显示出激活的表型. 然而,古典单细胞在这些炎症性疾病中表现出减少的激活标记.
科学领域:
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
背景情况:
- 巨细胞性关节炎 (GCA) 和多肌痛性风湿症 (PMR) 是相关的炎症状况.
- 抗原呈现细胞 (APC),包括单细胞和树突细胞 (DC),都与GCA和PMR病原发生有关.
- 在诊断GCA/PMR时,关于外周血液APC表型的信息有限.
研究的目的:
- 为了研究和比较APC表型在外周血液的未经治疗的GCA/PMR患者与健康对照.
- 分析不同单细胞和DC子集上的关键受体和标记物的表达.
主要方法:
- 流细胞计用于分析来自GCA/PMR患者的外周血液单核细胞 (PBMC) (n=15) 和年龄/性别匹配的健康对照 (HCs,n=15).
- 单细胞和DC子集 (pDCs,cDC1,cDC2) 被确定并分析了TLR2,TLR4,CD86,PDL1,CD40,HLA-DR和CD11c的表达.
- 在GCA患者的APC上进行了单细胞RNA测序.
主要成果:
- 在GCA/PMR患者和HC患者之间观察到APC的不同聚类.
- 在GCA/PMR患者中,单细胞子集比例变化和cDC1群体减少;cDC2比例与CRP负相关.
- 在GCA/PMR中的古典单细胞表现出减少的TLR2,HLA-DR和CD11c表达,与非古典单细胞的高表达形成鲜明对比.
结论:
- 在诊断时,循环的非古典单细胞在GCA/PMR患者中显示了一个激活的表型.
- 这些患者的古典单细胞显示激活标记物的表达减少.
- 需要进一步的研究来确定这些发现是否反映了APC迁移或慢性炎症效应.
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