一个纤维素纤维的直径是多少?
Heather A Belcher1, Martin Guthold2, Nathan E Hudson1
1Department of Physics, East Carolina University, Greenville, NC 27858, USA.
Research and practice in thrombosis and haemostasis
|August 21, 2023
概括
标准化纤维素纤维直径测量对于了解心脏病和中风等疾病至关重要. 这项研究比较了显微镜和度测量,发现SEM和超分辨率成像一致,为一致的纤维素结构分析铺平了道路.
科学领域:
- 生物物理学的生物物理.
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 改变的纤维素纤维结构与诸如冠心病,缺血性中风和动脉样硬化等心血管疾病有关.
- 目前研究纤维素结构的方法缺乏标准化,这阻碍了结果的直接比较.
研究的目的:
- 建立一条通向纤维素纤维直径测量标准化的道路.
- 使用多种实验技术,在各种纤维素和血栓度中比较纤维素纤维直径.
主要方法:
- 使用扫描电子显微镜 (SEM) 和超分辨率光显微镜 (SRM) 确定纤维素纤维直径.
- 采用了四种常见的流测量方法来确定直径.
- 在不同的生化条件下评估不同方法之间的一致性.
主要成果:
- 在大多数测试条件下,SEM和SRM的直径值在10%以内一致.
- 经过校正的度测量 (Yeromonahos方法) 显示在特定条件下 (500-800nm波长) 的SEM值在20%以内达成一致.
结论:
- 由于SEM和SRM之间的密切一致性,在SEM样本准备过程中,纤维素纤维截面的变化是最小的.
- 在定义条件下,通过SEM,SRM和度测量之间的一致性,证明了纤维素直径标准化项目的可行性.
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