LncRNA SATB2-AS1促进瘤生长和转移,并通过调节 SATB2 影响骨髓瘤中瘤免疫微环境
Peipei Wang1,2,3, Jianwei Zhu4,3, Qingqin Long2,3
1Department of Oncology, the Second Affiliated Hospital, and School of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, Guangdong 510180, PR China.
Journal of bone oncology
|August 21, 2023
概括
长非编码RNASATB2-AS1通过调节SATB2.2,促进骨髓瘤的生长和转移. 同时表达SATB2-AS1和SATB2表明预后不佳,可能作为治疗点.
科学领域:
- 分子瘤学分子瘤学
- 癌症生物学 癌症生物学
- 生物标志物 生物标志物
背景情况:
- 骨髓瘤是一种主要的骨恶性瘤,预后不佳.
- 长非编码RNASATB2-AS1在骨髓瘤中被上调,并在体外促进细胞增殖.
- 在骨髓瘤中SATB2-AS1的体内机制和预后意义尚不清楚.
研究的目的:
- 为了研究SATB2-AS1在骨髓瘤生长和活体转移中的作用.
- 评估SATB2-AS1和SATB2在骨肉瘤患者的预后价值.
- 阐明SATB2-AS1介导的SATB2.2调节背后的分子机制.
主要方法:
- 来自TARGET和GZFPH数据库的转录组测序数据分析.
- 在体内小鼠瘤模型评估瘤生长和转移.
- 双露西法酶记者基因测定,RNA拉下测定和生物信息学分析以探索分子机制.
主要成果:
- 增加SATB2-AS1和SATB2的共同表达与整体存活率差和无复发存活率明显相关.
- 在体内通过调节SATB2.2,SATB2-AS1促进骨髓瘤瘤的生长和肺转移.
- SATB2-AS1直接与POU3F1结合,以调节SATB2的表达,并可能通过IL-17信号通路调节免疫细胞透.
结论:
- SATB2-AS1通过激活SATB2促进骨肉瘤细胞生长和肺转移,与患者预后不佳相关.
- SATB2-AS1和SATB2是骨髓瘤风险分层的潜在生物标志物.
- SATB2-AS1和SATB2代表了骨髓瘤治疗的有前途的治疗点.
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