CCDC189通过与CABCOCO1相互作用,影响精子鞭毛的形成
Mengyue Wang1,2,3, Junyan Kang4, Zhiming Shen1,2,3
1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100020, China.
National science review
|August 21, 2023
概括
包含 189 (Ccdc189) 基因的卷曲-卷曲域对于精子鞭子的形成至关重要. 它的失活会导致由于异常的精子鞭毛导致男性不孕,这是多种形态异常的关键原因精子鞭毛 (MMAF).
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 细胞生物学 细胞生物学
背景情况:
- 精子鞭子 (MMAF) 的多重形态异常是男性不孕症的重要原因.
- 了解精子鞭状体形成的遗传和分子基础对于解决男性不孕症至关重要.
研究的目的:
- 为了研究含有189 (Ccdc189) 基因的卷曲-卷曲域在精子鞭子形成和男性生育能力中的作用.
- 确定与CCDC189相互作用的蛋白质,并阐明它们在精子发育中的功能.
主要方法:
- 在小鼠丸中的基因表达分析.
- 使用组织学和免疫电子显微镜对Ccdc189缺乏的小鼠的精子进行表型分析.
- 同免疫沉和质谱测量以确定CCDC189相互作用蛋白.
- 对Ccdc189及其相互作用伙伴在精子发育中的功能性研究.
主要成果:
- Ccdc189主要表达在小鼠丸中,其无活化会导致男性不孕.
- 缺少Ccdc189的精子表现出典型的MMAF表型,包括卷曲的,曲的或短的鞭毛.
- CCDC189蛋白位于精子轴名的辐射.
- 鉴定出CABCOCO1是一种CCDC189相互作用蛋白;其无活化也导致精子鞭毛形.
- CCDC189和CABCOCO1与辐射尖端蛋白RSPH1和内鞭状运输蛋白相互作用,而CCDC189的缺乏会降低CABCOCO1的表达.
结论:
- Ccdc189是一种辐射关联蛋白质,对于精子鞭子形成至关重要.
- CCDC189-CABCOCO1复合体在维持精子鞭毛结构和功能方面发挥着至关重要的作用.
- 在CCDC189介导途径中发生的干扰会导致与MMAF相关的男性不孕症.
相关概念视频
Mechanism of Filopodia Formation
2.4K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.4K
Cohesins
4.6K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.6K
Microtubules in Cell Motility
3.3K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
3.3K
Sperm Transport
923
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
923
Flagella and Motility in Bacteria
56
Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
56
Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K


