使用Trichoderma reesei和Coprinus comatus进行Codonopsis pilosula的混合真菌发酵的反应表面方法
Ti Wei1,2, Hongfu Chen1,2, Dengyu Wu2,3
1College of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, China.
PeerJ
|August 21, 2023
概括
使用Trichoderma reesei和Coprinus comatus的Codonopsis pilosula (CPS) 的混合真菌发酵有效降解纤维素和素. 最佳条件产生了显著的降解率,提高了料质量.
科学领域:
- 农业科学 农业科学
- 生物技术是生物技术.
- 微生物学 微生物学
背景情况:
- 草Codonopsis pilosula (CPS) 呈现出一种具有动物料潜力的纤维纤维素生物质.
- 有效降解纤维素和红素对于提高CPS营养价值至关重要.
- 混合真菌发酵为生物质价值化提供了一个有前途的方法.
研究的目的:
- 为了确定CPS的纤维素降解率 (CDR) 和素降解率 (LDR).
- 确定CPS混合真菌发酵的最佳发酵参数.
- 建立一个用于预测退化速度的数学模型.
主要方法:
- 进行了单因素实验,以评估真菌比率 (Trichoderma reesei:Coprinus comatus),注射剂大小,玉米面粉含量和发酵时间的影响.
- 使用响应表面方法来优化发酵参数和分析因子相互作用.
- 使用设计专家软件v8.0.6.6开发了一个二次多项式模型.
主要成果:
- 最佳的发酵参数包括真菌比率为4:6:6,8%的真菌注射剂,10%的玉米面粉含量和15天的发酵时间.
- 在最佳条件下,CDR达到13.65%,LDR达到10.73%.
- 混合的真菌发酵导致纤维素和红素含量降低,营养素保留率提高,发酵质量提高.
结论:
- 用Trichoderma reesei和Coprinus comatus发酵是一种有效的降解CPS草的方法.
- 该研究为利用发酵CPS作为一种有价值的动物料资源提供了理论基础.
- 优化的发酵参数显著提高了纤维素生物质的降解效率.
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