在卫星RNA的实验进化过程中,局部复制和远距离传播之间的权衡
Shu-Chuan Lee1, Ming-Ru Liou2, Yau-Heiu Hsu2
1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
Frontiers in microbiology
|August 21, 2023
概括
卫星RNAs (satRNAs) 通过其5'未翻译区域 (UTR) 的突变进化. 这项研究揭示了病毒RNA复制和系统运动之间的权衡,影响了satRNA进化.
科学领域:
- 植物病毒学 植物病毒学
- 分子进化分子进化
- 在RNA生物学,RNA生物学.
背景情况:
- 卫星RNAs (satRNAs) 是依赖于辅助病毒进行复制的RNA分子.
- 之前的研究集中在satRNAs的分子和遗传学方面,缺乏实验进化数据.
- 了解感染条件下的satRNA进化是至关重要的.
研究的目的:
- 在不同的感染场景下调查竹马赛克病毒相关的satRNA (satBaMV) 的实验进化.
- 在串行传递过程中识别satBaMV的突变和种群动态.
- 探索satBaMV复制和宿主植物传播之间的关系.
主要方法:
- 在共感染和超级感染条件下,satBaMV在*Nicotiana benthamiana*植物中的连续传输.
- 深度测序以识别satBaMV群体中的单核酸多态 (SNP).
- 竞赛实验使用原生质和整个植物中的工程 satBaMV 菌株.
主要成果:
- 确定了48个SNP,其中大多数是治疗特异性的,而5' UTR中的位置30,34,63和82的突变是普遍的.
- 全球SNP形成了具有明显人口动态的单元类型.
- 在本地复制 (高原生体/注射叶数) 和远距离传播 (高系统叶数) 之间观察到一个权衡.
结论:
- 在satBaMV的5' UTR中发生的普遍突变影响了它的进化.
- 复制和传播之间明显的权衡,可能是由于对抗性类型,影响了satRNA运动.
- 这项研究强调了satRNA复制和系统运动之间的未被探索的联系,这对于理解satRNA进化至关重要.
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