Vidjil附加程序用于使用EuroClonality-NGS协议处理的样本的MRD量化
Guilherme Navarro Nilo Giusti1,2, Antonio Vítor Ribeiro3, Patrícia Yoshioka Jotta1
1Centro de Pesquisa Boldrini Centro Infantil Boldrini Campinas São Paulo Brazil.
EJHaem
|August 21, 2023
概括
一个新的Vidjil附加组件可以分析EuroClonality-NGS库,以进行急性淋巴细胞白血病的最小残留疾病评估. 这种开源解决方案提供与现有的封闭源代码软件高度一致的结果.
科学领域:
- 免疫学 免疫学 免疫学
- 生物信息学是一种生物信息学.
- 在瘤学瘤学.
背景情况:
- 在急性淋巴细胞白血病 (ALL) 中精确评估最小残留疾病 (MRD) 对治疗分层至关重要.
- 免疫谱的下一代测序 (NGS),特别是CDR3序列,是检测MRD的强大工具.
- 欧洲Clonality-NGS协议提供了一个全面的方法来准备MRD评估库,但Vidjil等现有的开源工具无法处理它们.
研究的目的:
- 开发一个与ALL中MRD评估的EuroClonality-NGS协议兼容的开源解决方案.
- 增强Vidjil软件处理使用EuroClonality-NGS方法编制的库的能力.
- 为了验证Vidjil附加组件的性能与已知的封闭源代码软件相比.
主要方法:
- 一个Vidjil附加组件的开发.
- 使用新的Vidjil附加程序和ARResT/Interrogate软件处理EuroClonality-NGS准备的样本.
- 对两种软件平台获得的结果进行比较分析.
主要成果:
- 维吉尔附加程序成功处理了使用EuroClonality-NGS协议编制的库.
- 对EuroClonality-NGS样本的分析显示,Vidjil和ARResT/Interrogate之间存在很高的一致性 (r = 0.998).
- 维吉尔附加程序显示出较低的错误率 (RMSE = 0.112),表明可靠的性能.
结论:
- 开发的Vidjil附加程序提供了一个可行的开源替代方案,用于分析EuroClonality-NGS库用于ALL的MRD评估.
- 这一进步提高了研究人员和临床医生的先进MRD检测方法的可访问性.
- 高一致性和低误差证实了Vidjil附加组件对该应用程序的可靠性.
关键词:
T细胞受体受体的 T 细胞受体.维吉尔·维吉尔·维吉尔 (英语:Vidjil Vidjil) 是一个在线视频.免疫系统的曲目.免疫球蛋白基因 免疫球蛋白基因这种白血病是白血病.最少残留疾病的最小残留疾病.更多相关视频
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