相关实验视频

Updated: Jul 18, 2025

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

TeloBase:一个社区策划的生命树上的端粒序列数据库

Martin Lyčka^1,2, Michal Bubeník², Michal Závodník^1,2

¹Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, BrnoCZ-62500, Czech Republic.

Nucleic acids research

|August 21, 2023

概括

TeloBase是一个全新的综合数据库,详细介绍了整个自然界的端粒DNA模式多样性. 它通过整合文献和下一代测序数据来解决现有资源的局限性,确保研究人员获得最新的信息.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

最近的发现突显出各种生物体中端粒DNA图案的显著多样性.
现有的数据库,如"Telomerase数据库"和"植物rDNA数据库",由于重点不同和更新不频繁,提供不完整的覆盖范围.
需要一个全面的,最新的资源来对端粒DNA模式多样性进行分类.

研究的目的:

创建一个全面的数据库,TeloBase,用于端粒DNA模式多样性.
为了促进数据过和演化动态的可视化.
建立一个社区策划系统,以持续更新.

主要方法:

广泛的文献审查,以收集现有的端粒DNA序列数据.
对公开可用的下一代测序 (NGS) 数据的分析.
为TeloBase.开发一个内部分类和一个用户友好的社区策划系统.
端粒酶RNA模板区域的生物信息分析.

主要成果:

TeloBase被建立为一个对端粒DNA模式信息的全面存储库.
数据库包含内部分类系统,用于数据过和演化动态的可视化.
一个新的端粒基因, (TTTATTAGGG) n,在植物家族Chrysobalanaceae中被确定.
研究了真菌属Aspergillus中的端粒基因多样性.

结论:

TeloBase有效地解决了端粒DNA图案数据中存在的差距.
数据库的社区策划系统确保了它的及时性和相关性.
使用TeloBase. 新的端粒图案和进化见解可以快速发现和共享.

更多相关视频

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

相关概念视频

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

The Tree of Life - Bacteria, Archaea, Eukaryotes

The Tree of Life - Bacteria, Archaea, Eukaryotes

The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...