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康尼辛43和细胞培养基质差异调节OCY454骨细胞分化和向主要骨细胞发送信号

Gabriel A Hoppock¹, Evan G Buettmann¹, Joseph A Denisco¹

¹Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia, United States.

American journal of physiology. Cell physiology

|August 21, 2023

概括

骨细胞中Connexin 43 (Cx43) 缺乏会独立促进骨重塑. 三维培养增强了骨细胞分化和骨重塑因子分泌,在研究Cx43缺乏的骨细胞机制方面表现优越.

科学领域:

骨生物学骨生物学骨生物学
蜂通信蜂通信
骨的恒常状态骨的恒常状态

背景情况:

连接素43 (Cx43) 是骨中关键的隙间连接蛋白,对细胞通信和骨健康至关重要.
骨细胞特异性的Cx43删除与改变的骨形成和再吸收有关,但其在骨重塑中的细胞自主作用尚不清楚.
三维 (3D) 培养可以增强骨细胞表达和分泌骨重塑因子,如硬质素和RANKL.

研究的目的:

为了研究骨质细胞Cx43在骨重塑中的细胞自主作用.
为了比较2D与3D培养系统对骨细胞表型和功能的影响.
确定Cx43缺乏对骨细胞分化和骨改造因子分泌的影响.

主要方法:

OCY454骨细胞,无论是野生型 (WT) 还是Cx43淘汰 (Cx43KO),都是在2D和3DAlvetex支架上培养的.
对条件介质进行了可溶性信号因子分析.
主要骨髓细胞使用条件介质分化为骨质母细胞和骨质母细胞.

主要成果:

与2D培养相比,3D培养促进了成熟的骨质细胞表型,基因表达增加,扩散减少.
在3D培养中,Cx43缺乏并没有显著改变骨细胞分化.
与Cx43KO细胞相比,3D培养的WT细胞显示了增加的胆固醇分泌.
来自Cx43KO细胞的条件介质,特别是来自3D培养的介质,增强了骨质母细胞发生和骨质母细胞发生.

结论:

骨细胞Cx43缺乏促进细胞自主的方式骨重塑,对骨细胞分化的影响最小.
三维培养系统对于研究Cx43缺乏的骨细胞,促进分化和增强骨改造因子分泌的优越性.
这些发现强调了Cx43在调节骨重塑中的重要性以及3D培养模型的实用性.

关键词:

三维细胞培养的3D细胞培养连接器 43 连接器 43 差距连接点差距连接点差距连接点在体外 (in vitro) 进行试验.这些骨质细胞是骨质细胞.

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