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合成和生物分析的新型斯特里戈拉克衍生物用于Arabidopsis的生长和发育

Zhaoyong Kang¹, Yujie Yan¹, Ruirui Lu²

¹School of Pharmaceutical Science and Technology, Institute of Molecular Plus, Frontiers Science Center for Synthetic Biology (Ministry of Education of China), Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, P. R. China.

Journal of agricultural and food chemistry

|August 21, 2023

概括

研究人员合成了新型的strigolactone (SL) 类似物,以研究植物发育. 一些化合物模仿GR24的作用.

科学领域:

植物生物学植物生物学
化学生物学化学生物学
分子生物学分子生物学

背景情况:

斯特里戈拉克顿 (SLs) 是重要的植物激素,调节各种发育过程.
合成模拟物GR24是SL研究中广泛使用的参考化合物.
了解结构-活动关系是发现新型SL衍生品的关键.

研究的目的:

为了设计和合成新型的strigolactone衍生物.
探索这些新化合物在Arabidopsis中的结构-活性关系.
确定在植物发展中具有独特或增强活动的SL类型.

主要方法:

三系列新型GR24衍生物的化学合成.
对合成化合物的测定对 *Arabidopsis* 中的 hypocotyl 和根部发育的影响.
分子动力学模拟以调查连接体-受体相互作用.

主要成果:

合成和测试了50种新的SL类似物.
化合物A11a,A12a和A20d表现出高活性,与GR24相比,在抑制皮和根延长方面具有较高活性.
确定了特定的抑制剂 (A20c,A21e,A21o),促进剂 (A21c,A26c,A27a) 和一种具有与GR24相反作用的独特化合物 (A27e).
AtD14受体没有影响SL类似物对根部发育的抑制作用.
分子动力学模拟提供了对类似物A11a和A27e的独特功能的见解.

结论:

发现了具有多样性活动的新型strigolactone类似物,包括抑制,促进和对植物发育具有独特的对抗作用.
分子动力学模拟阐明了独特的联体受体相互作用的基础,有助于设计新型植物激素.
澄清了AtD14受体在调解SL模拟效应对根部发育中的作用.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.GR24的同类产品是GR24.植物激素植物激素排球菌的作用是什么?结构 - 活动关系关系.

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