双向多细胞挤出由Notch驱动的基底挤出和Piezo1驱动的顶端挤出控制
Rosa Ventrella1,2, Sun K Kim1, Jennifer Sheridan1
1Northwestern University, Feinberg School of Medicine, Department of Cell and Developmental Biology, Chicago, IL 60611, USA.
Development (Cambridge, England)
|August 21, 2023
概括
两个不同的机制,通过Notch信号的基底挤出和通过Piezo1的尖端挤出,在Xenopus胚胎发育过程中驱动多细胞 (MCC) 损失,年龄依赖的调节会影响上皮质重塑.
科学领域:
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
- 皮质生物学 皮质生物学
背景情况:
- 克塞诺普斯胚胎具有复杂的上皮质,含有丰富的多细胞 (MCC).
- 在晚期发育过程中表皮质重塑涉及到MCCs的完全丧失.
- 细胞挤出是细胞去除的关键机制,维护了上皮质完整性.
研究的目的:
- 调查在Xenopus胚胎发育过程中多细胞 (MCC) 挤出背后的独特机制.
- 阐明Notch信号和Piezo1在调节MCC损失中的作用.
- 了解细胞挤出过程的年龄相关调节.
主要方法:
- 对Xenopus胚胎发育的观察.
- 细胞挤出动态的分析.
- 对信号通路 (Notch) 和机械传感器 (Piezo1) 的研究.
- 与MCC维持相关的基因表达的评估.
主要成果:
- 确定了两个不同的MCC挤出机制:基底挤出 (依赖Notch) 和顶端挤出 (依赖Piezo1).
- 观察到从基底到顶端挤出的发育转变.
- 痕信号响应是年龄相关的,与MCC转录程序相关,提供对细胞损失的保护.
- Piezo1 调节顶端挤出,其激活或抑制会影响 MCC 挤出时间和维护.
结论:
- 在上皮重塑过程中,MCCs的去除是通过不同的,受调节的挤出机制实现的.
- 年龄依赖的信号通路,包括Notch和Piezo1,控制MCC挤出.
- 了解这些机制对于理解上皮细胞发育和重塑过程至关重要.
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