莱克托巴西勒斯 (Lactobacillus rhamnosus) 通过抑制TLR2/NF-κB通路来抑制骨质细胞分化,从而抑制骨质细胞分化
Jingfei Fu1, Lu Jia1, Lili Wu1
1Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.
Oral diseases
|August 21, 2023
概括
超声波 Lactobacillus rhamnosus 提取物通过关闭关键信号通路来抑制牙周炎中的骨质细胞分化和膜骨损失. 这为治疗这种炎症性牙疾病提供了一个新的益生菌策略.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 牙周病学 牙周病学
背景情况:
- 牙周炎涉及过度的骨质细胞活动,导致膜骨的破坏.
- 拉科巴西勒斯 (Lactobacillus rhamnosus) 是一种具有潜在免疫调节作用的益生菌.
- 向骨质细胞生成是牙周炎治疗的关键策略.
研究的目的:
- 为了研究超声波Lactobacillus rhamnosus提取物对骨质细胞分化的影响.
- 为了阐明底层的分子机制.
- 在牙周炎小鼠模型中评估其治疗潜力.
主要方法:
- 由M-CSF和RANKL引起的骨质细胞分化.
- 使用超声波和超离心法制备的 Lactobacillus rhamnosus 提取物.
- 用于通过TLR2.2分析信号通路 (NF-κB/c-Fos/NFATc1) 的西方抹杀方法.
- 用局部提取物注射建立的小鼠牙周炎模型.
主要成果:
- 乳杆菌拉姆诺索斯提取物 (50微克/毫升) 抑制了骨质细胞分化,但没有影响亡或增殖.
- 提取物使NF-κB/c-Fos/NFATc1信号通路通过类似收费受体2失活.
- 局部注射的提取物抑制了骨质细胞分化和膜骨再吸收 in vivo.
结论:
- 超声波 Lactobacillus rhamnosus 提取物有效抑制骨质细胞分化.
- 这种机制涉及抑制NF-κB/c-Fos/NFATc1通路.
- 这个提取物呈现了一种有前途的新策略,用于使用益生菌治疗牙周炎.
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