由于膜电位而增加离子Fe3O4纳米粒子诱导的膜孔和囊泡变形 - - 一项实验研究
Md Moniruzzaman1, Mohammad Abu Sayem Karal1, Md Abdul Wadud1
1Department of Physics, Bangladesh University of Engineering and Technology, Dhaka-1000, Bangladesh. asayem221@phy.buet.ac.bd.
Physical chemistry chemical physics : PCCP
|August 21, 2023
概括
膜潜力显著影响纳米粒子与脂质囊泡的相互作用. 这项研究表明,负膜潜能增强纳米粒子诱导的细胞模拟囊泡中的孔隙和变形.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 细胞生物学 细胞生物学
背景情况:
- 膜潜能对于细胞过程和与膜活性剂的相互作用至关重要.
- 之前关于纳米粒子-脂质囊泡相互作用的研究缺乏膜潜力考虑.
- 了解这些相互作用对于药物输送和纳米医学至关重要.
研究的目的:
- 为了研究膜潜力的作用对阳离子磁铁纳米颗粒诱导的孔隙和细胞模拟巨型单囊 (GUVs) 的变形.
- 探索不同的膜潜能如何影响纳米粒子 - 囊泡相互作用和膜透性.
主要方法:
- 使用脂质1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DOPG),1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) 和格拉米西丁A (GrA) 合成巨型单囊 (GUVs) 的方法.
- 使用相对照光显微镜研究GUV的静态和动态性质.
- 在不同的膜电位下分析来自GUV的素泄漏,以确定孔隙和变形速率.
主要成果:
- 格拉米西丁A (GrA) 的存在减少了在没有膜潜力的情况下的泄漏常数.
- 增加负膜潜力将素泄漏从单个指数函数转移到双指数函数,表明透动态发生了变化.
- 囊泡孔隙和变形都随着阴性膜潜力的增加而增加.
- 泄漏动力学显示,在较高负潜力下,初始速率更快,最终速率更慢.
结论:
- 膜潜力显著增强了GUV中的纳米粒子诱导的孔隙和变形.
- 在更高的负潜力下,纳米颗粒和膜之间的结合亲和力增加是推动增强膜透和囊泡变形的关键因素.
- 这些发现为在生理条件下的纳米粒子-膜相互作用提供了关键的见解.
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