相关实验视频
Updated: Jul 18, 2025

07:47
Microscopy of Fission Yeast Sexual Lifecycle
Published on: March 9, 2016
14.6K
振荡式生长的同步准备了真菌菌体的融合
Valentin Wernet1, Marius Kriegler1, Vojtech Kumpost2,3
1Karlsruhe Institute of Technology - South Campus Institute for Applied Biosciences Dept. of Microbiology, Karlsruhe, Germany.
eLife
|August 21, 2023
概括
菌在融合之前使用细胞独白进行交流. 信号蛋白质Soft (SofT) 和MAK-2 (MakB) 在这个独白过程中相位振荡,然后在细胞对话中转换为反相位振荡以进行合.
科学领域:
- 菌类学 菌类学是指菌类学.
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 依赖于分泌的信号分子进行细胞间通信,协调融合和状细胞网络的形成.
- 交互的叶表现出信号发送和接收的交替模式,由软 (SofT) 和MAK-2 (MakB) 蛋白振荡可视化.
- 之前的研究集中在细胞间的对话,但单个字体内的沟通仍然未被探索.
研究的目的:
- 在没有融合伙伴的情况下,研究单个真菌菌中的信号振荡.
- 描述不同通信阶段的软 (SofT) 和MAK-2 (MakB) 振荡的动态.
- 探索离子 (Ca2+) 在真菌沟通和菌融合中的作用.
主要方法:
- 在Arthrobotrys flagrans*单片中观察软 (SofT) 和MAK-2 (MakB) 蛋白的振荡.
- 数学建模以模拟共享的细胞外环境中的信号动态.
- 测量细胞内Ca2+度,并评估Ca2+耗尽对状动物行为的影响.
主要成果:
- 单个体表现出"细胞独白"与同步的软 (SofT) 和MAK-2 (MakB) 振荡与生长振荡相一致.
- 接近的叶经历了引进,在实现反相同步之前减缓振荡频率.
- 在接近叶时观察到细胞内Ca2+振荡;Ca2+耗尽扰乱了单音和反相同步.
结论:
- 菌叶参与环境探索的"细胞独白".
- 这种独白会动态地转变为"对话",以启动合.
- 离子在调节真菌沟通和融合过程中起着至关重要的作用.
相关概念视频
Yeast Signaling
14.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.7K
Diversity of Protists IV
37
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
37
Fungal Group Zygomycota
42
Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
42
Cells Coordinate Growth and Proliferation
4.5K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.5K
Overview of Fungi
54
Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...
54
Gene Regulation During Sporulation
34
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
34

