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在大豆子中的一个氨酸受体的分子特征和表达模式,Chrysodeixis includensens

Sena Isbilir¹, Beverly Catchot¹, Lauren Catchot¹

¹Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, USA.

Archives of insect biochemistry and physiology

|August 21, 2023

概括

大豆循环表现出适度的耐药性,以二胺杀虫剂的兰地利. 这项研究对大豆循环氨酸受体 (RyR) 基因进行了测序,揭示了其结构和表达模式,提供了对抗虫剂耐药性机制的见解.

科学领域:

分子昆虫学分子昆虫学
抗虫剂耐药性抗虫剂耐药性抗虫剂耐药性
农业学是一种农业学.

背景情况:

胺杀虫剂针对昆虫的氨酸受体 (RyRs) 进行害虫控制.
增加昆虫耐药性需要了解关键害虫中的RyR分子结构.
大豆虫 (Chrysodeixis includens) 是一个重要的作物害虫,具有非特征的胺耐药性.

研究的目的:

为了研究金刚石在Chrysodeixis includens中对杀虫剂的耐药性.
描述C.includens的分子结构和基因表达,包括氨酸受体 (RyR).
为了探索潜在的分子机制,这种物种的底层胺耐药性.

主要方法:

全长C.的测序包括RyRcDNA.
基因组分析以确定基因结构 (外原体,替代拼接).
将C.includens的RyR序列与其他昆虫的RyR序列进行比较.
查已知与耐药性相关的突变.
通过定量PCR在不同幼虫阶段和组织中进行基因表达分析.
评估RyR表达在亚致死的中利露暴露后.

主要成果:

在C.includens.现场种群中检测到中度的兰特兰尼利醇耐药性.
包含CincRyR基因 (CincRyR) 的CincRyR基因包括113个外显子,编码具有保留功能域的大型蛋白质.
在查的种群中没有发现已知的胺抗性突变.
在第三阶段幼虫中,RyR的表达很高,特别是在肌肉组织中.
在96小时后,亚致命的兰特利烯暴露降低了RyR表达水平.

结论:

这项研究提供了C. includens RyR. RyR. 的第一个分子特征.
这些发现为了解大豆循环器中的二胺抗性机制奠定了基础.
需要进一步的研究来阐明驱动观察到的抗性的特定机制.

关键词:

克里索德伊克西斯包括包括.兰地利烯 (Chlorantraniliprole) 是一种酸盐.这是一种二氧化,二氧化,二氧化.基因表达的基因表达方式氨酸受体是氨酸的受体.豆的循环器豆的循环器

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