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Updated: Jul 18, 2025

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
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纯化和检测单核酸KRAS突变在人体血中染色体外圆形DNA上的方法

Lasse Bøllehuus Hansen1, Sandra Fugl Jakobsen1, Egija Zole1

  • 1Department of Biology, University of Copenhagen, Copenhagen, Denmark.

Cancer medicine
|August 21, 2023
PubMed
概括

这项研究比较了eccDNA净化方法,发现固相净化更有效. 目前的qPCR方法缺乏用于癌症诊断的血eccDNA中KRAS突变检测的灵敏度.

关键词:
在KRAS突变中发生突变.圆形的 DNA 圆形的 DNA这就是为什么eccDNADNA.液体活检活检液体活检醇/甲基 DNA 提取方法塑料体中的塑料.

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