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Updated: Jul 18, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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最近的进展和最佳实践对于用于蛋白质结构确定的冷电磁分析

Julien Bergeron1, Chiara Rapisarda2

  • 1Randall Centre for Cell and Molecular Biophysics, King's College London; julien.bergeron@kcl.ac.uk.

Journal of visualized experiments : JoVE
|August 21, 2023
PubMed
概括

本系列文章详细介绍了为电子显微镜准备样本的先进技术. 创新包括机器人系统和冷电子显微镜的新方法,改进数据采集和分析.

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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