强化能源消耗促进微生物细胞外呼吸,用于异生生物生物转化
Zi-Han Liang1, Hong Sun2, Yang Li2
1Department of Environmental Science and Technology, University of Science and Technology of China, Hefei, China.
Environmental microbiology
|August 21, 2023
概括
这项研究引入了一种新的策略,通过操纵细胞能量水平来增强微生物细胞外电子转移 (EET),从而改善异生生物的生物降解. 控制ATP水解以增强细胞外呼吸 (CHEER) 方法提高了环境修复中的微生物效率.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 电原体利用细胞外电子转移 (EET) 进行异生物生物转化.
- 合成生物工程增强了EET,但传统方法由于复杂的代谢调节而面临限制.
- 需要一种新的,路径独立的方法来改善ETE输出.
研究的目的:
- 开发和评估一种新的战略,通过能量操纵来增强微生物ETE输出.
- 研究ATP受控化以增强细胞外呼吸 (CHEER) 策略对电源微生物的影响.
- 评估CHEER战略对异生菌生物转化效率的影响.
主要方法:
- 开发了ATP的受控化以增强细胞外呼吸 (CHEER) 策略.
- 将 CHEER 策略应用于 Shewanella oneidensis,这是一个模型电源细菌.
- 利用微生物电解电池和微生物燃料电池来评估ETT能力.
- 进行转录组分析以了解代谢变化.
- 评估了甲基,CrVI和UVI的生物转化效率.
主要成果:
- CHEER策略有效地降低了细胞内ATP水平,加速了碳基质消耗和生物质积累.
- 改进后的CHEER菌株在微生物燃料电池和微生物电解细胞中显著提高了ET的能力.
- 转录组分析显示,CHEER菌株的生物质合成和代谢活性增加.
- 使用CHEER菌株观察到甲基色,CrVI和UVI的提升去除效率.
结论:
- CHEER战略提供了一种新的,以能源为中心的方法来增强微生物EET,而不依赖于特定的基因或途径修改.
- 这一策略导致在异生生物生物转化中改善微生物的性能.
- 这些发现为促进微生物EET用于环境应用提供了新的视角和可行的方法.
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