对Helicobacter pylori感染的综合研究:分子分析,抗菌敏感性和组织病理学检查
Fatih Buyuk1, Emre Karakaya2, Mustafa Akar3
1Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. fatihbyk08@hotmail.com.
Antonie van Leeuwenhoek
|August 21, 2023
概括
杆菌感染在成年消化不良患者中很普遍,显示出显著的遗传多样性和对常见抗生素 (如克拉里胺和甲尼达) 的高耐药性. 这凸显了需要更新区域治疗指南,以有效地根除H. pylori.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 传染性疾病 传染性疾病
背景情况:
- 杆菌 (H. pylori) 是全球胃肠道疾病的主要原因之一.
- 了解H. pylori的患病率,基因型特征和抗生素敏感性对于有效的治疗策略至关重要.
研究的目的:
- 通过使用侵入性诊断方法,确定H. pylori在成年消化不良患者中的流行率.
- 分析H. pylori分离的抗生素敏感性和遗传特征.
- 为了将H. pylori基因型与胃活检中的组织病理学发现相关联.
主要方法:
- 一项涉及208名成年消化不良患者的横截面研究.
- 用培养物,组织聚合酶链反应 (PCR) 和组织病理学进行胃活检分析.
- 通过梯度方法进行抗生素敏感性测试,并通过单复式PCR进行病毒性基因分析.
- 使用肠杆菌重复性基因间共识PCR (ERIC-PCR) 进行的基因型鉴定.
主要成果:
- 总的来说,H. pylori的流行率为63%,通过组织PCR (54.8%) 的检测率高于培养 (28.4%) 或组织病理学 (38.9%).
- 对甲尼达 (67.2%),澄素 (27.9%) 和叶黄素 (34.4%) 观察到高耐药率,其中45.9%具有多药耐药性 (MDR).
- 发现了显著的基因型异质性,其中cagga是最常见的毒性基因 (93.44%),并确定了26种不同的ERIC-PCR模式.
结论:
- 在成年消化不良患者中,H. pylori感染的发病率很高,与发展中国家的研究结果一致.
- 这项研究揭示了H. pylori分离物中显著的基因型多样性和毒性基因变异性.
- 高抗生素耐药性需要仔细考虑区域实证治疗方案,以消除H. pylori.
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