雌激素调节在子宫内膜上皮细胞中通过乙蛋白硫酶 (APT) 介导的S-棕化在腺菌症中的纹局部化
Zhixing Jin1, Juan Wang2, Youguo Chen3
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
Reproductive sciences (Thousand Oaks, Calif.)
|August 21, 2023
概括
雌激素影响在腺肌病中Scribble蛋白的局部化,这是疾病发展的关键因素. 这项研究揭示了雌激素如何通过S-palmitoylation影响Scribble蛋白,为腺菌病原体提供了新的见解.
科学领域:
- 妇科 妇科医生 妇科
- 细胞生物学 细胞生物学
- 分子病原体的产生.
背景情况:
- 尽管腺菌的流行率和症状严重程度,但腺菌的发病因子和病因仍然不明确.
- 以前已经观察到Scribble蛋白的部分细胞质转移,但其调节机制尚不清楚.
- 子宫内膜中超生理性雌激素的产生与腺菌症的发展有关.
研究的目的:
- 为了研究雌激素对腺肌病中Scribble蛋白局部化的作用和机制.
- 阐明S-palmitoylation在雌激素介导的Scribble调节中的作用.
主要方法:
- 在体内和体外研究分析了Scribble局部化在女性和小鼠腺菌症.
- 用S-palmitoylation抑制剂 (2-bromopalmitate) 和雌激素受体抑制剂 (ICI182,780) 的治疗.
- 技术包括免疫光,西部斑点,乙-生物素交换棕化试验和免疫组织化学 (IHC).
主要成果:
- 与对照组 (27%的女性,10%的小鼠) 相比,在腺菌组织 (68%的女性,60%的小鼠) 中,Scribble转移到细胞质显著更高.
- 雌激素暴露诱导了Scribble细胞质转位和降低了S-palmitoylated Scribble (palm-Scribble) 水平,其影响可通过ICI182,780.80逆转.
- 雌激素通过APT介导的S-palmitoylation调节Scribble蛋白的Scribble局部化.
- 在人类腺菌组织中,APT1和APT2的表达显著增加,与芳酶P450.0正相关.
结论:
- 雌激素在通过S-palmitoylation调节Scribble蛋白的局部化方面发挥着至关重要的作用.
- 腺菌症中APT1和APT2表达的增加表明它们参与了疾病的发病.
- 这项研究提供了对腺菌病原的新理解,突出了雌激素-Scribble-APT通路.
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