来自海洋衍生的Streptomyces sp. 的结构多样化的代谢物. DS-27 基于两个不同的文化条件
Feihang Yan1, Jiebin Fang1, Wanjing Ding1,2
1Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, Zhoushan, 316021, China.
Chemistry & biodiversity
|August 21, 2023
概括
研究人员从海洋带绳草细菌中发现了9种新化合物,包括链杆菌素AE. 两种化合物通过抑制氧化生产,显示出潜在的抗炎作用.
科学领域:
- 自然产品化学 自然产品化学
- 海洋微生物学 海洋微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 海洋微生物,特别是来自Spartina alterniflora的微生物,是新型生物活性化合物的丰富来源.
- Streptomyces 属以产生各种具有制药潜力的二次代谢产物而闻名.
研究的目的:
- 从Streptomyces sp.中分离和描述新的化学实体. DS-27是一种源自海洋草的菌株.
- 评估分离化合物的生物活性,重点关注它们的抗炎性质.
主要方法:
- 使用广泛的1D/2D NMR和HR-ESI-MS数据进行结构阐明.
- 通过NOESY,ECD,特定旋转,GIAO NMR计算和DP4+分析来确定绝对配置.
- 生物活性查,包括对脂聚糖 (LPS) 诱导的氧化 (NO) 生产的抑制作用的评估.
主要成果:
- 隔离和识别了九种新的化合物:链杆菌胺 A-E (1-5),两个环素 (6, 7),一个α-pyrone (8),和wailupemycin Q (20).
- 同一个细菌菌株中还发现了16种已知的化合物.
- 化合物5和7显示显著的,剂量依赖的抑制LPS诱导的NO生产.
结论:
- 海洋衍生型的Streptomyces sp. 在海洋中被发现. DS-27是结构多样化的自然产品的丰富来源.
- 化合物5和7具有显著的抗炎作用,需要进一步研究治疗应用.
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